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A distinct class of slow (⁓0.2-2 Hz) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex

Citation

Lorincz, ML and Gunner, D and Bao, Y and Connelly, WM and Isaac, JT and Hughes, SW and Crunelli, V, A distinct class of slow (⁓0.2-2 Hz) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex, Journal of Neuroscience, 35, (14) pp. 5442-5458. ISSN 0270-6474 (2015) [Refereed Article]


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Copyright 2015 Lo˝rincz et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1523/JNEUROSCI.3603-14.2015

Abstract

During sleep and anesthesia, neocortical neurons exhibit rhythmic UP/DOWN membrane potential states. Although UP states are maintained by synaptic activity, the mechanisms that underlie the initiation and robust rhythmicity of UP states are unknown. Using a physiologically validated model of UP/DOWN state generation in mouse neocortical slices whereby the cholinergic tone present in vivo is reinstated, we show that the regular initiation of UP states is driven by an electrophysiologically distinct subset of morphologically identified layer 5 neurons, which exhibit intrinsic rhythmic low-frequency burst firing at 0.22 Hz. This low-frequency bursting is resistant to block of glutamatergic and GABAergic transmission but is absent when slices are maintained in a low Ca 2 medium (an alternative, widely used model of cortical UP/DOWN states), thus explaining the lack of rhythmic UP states and abnormally prolonged DOWN states in this condition. We also characterized the activity of various other pyramidal and nonpyramidal neurons during UP/ DOWN states andfoundthat an electrophysiologically distinct subset of layer 5 regular spiking pyramidal neuronsfires earlier duringthe onset of network oscillations compared with all other types of neurons recorded. This study, therefore, identifies an important role for cell-type-specific neuronal activity in driving neocortical UP states.

Item Details

Item Type:Refereed Article
Keywords:Sleep, Oscillations
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
UTAS Author:Connelly, WM (Dr William Connelly)
ID Code:131558
Year Published:2015
Web of Science® Times Cited:23
Deposited By:Medicine
Deposited On:2019-03-22
Last Modified:2019-04-29
Downloads:3 View Download Statistics

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