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TNF may negatively regulate phagocytosis of Devil Facial Tumour Disease cells by activated macrophages
Li, X and Darby, J and Lyons, AB and Woods, GM and Korner, H, TNF may negatively regulate phagocytosis of Devil Facial Tumour Disease cells by activated macrophages, Immunological Investigations, 48, (7) pp. 691-703. ISSN 0882-0139 (2019) [Refereed Article]
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© 2018 Taylor & Francis. This is an Accepted Manuscript of an article published by Taylor & Francis in Immunological investigations on 19/1/19, available online: http://www.tandfonline.com/10.1080/08820139.2018.1515222
Methods: We used Devil Facial Tumour Disease (DFTD) cancer cells as the target tumour cells. The Tasmanian devil (Sarcophilus harrisii) population is threatened by the transmissible DFTD. Using DFTD cells provided the opportunity to determine if these cells can be phagocytosed and investigate requirement for TNF. As effector cells, bone marrow derived macrophages (BMDMs), generated from C57BL/6 wild type (B6.WT) and C57BL/6 TNF-/- (B6.TNF-/-) mice were used. Phagocytosis of DFTD cells was investigated by confocal microscopy and flow cytometry.
Results: DFTD cells were consistently phagocytosed by B6.WT and B6.TNF-/- BMDMs with similar efficiency in vitro. Consequently the DFTD cells are not resistant to phagocytosis. Following activation by exposure to IFNγ and LPS or LPS alone, B6.TNF-/- BMDMs had higher phagocytic efficiency and lower nitric oxide (NO) production compared to wild-type controls. In addition, NO seems to be unlikely to be the involved in phagocytosis efficiency in IFNγ and LPS activated B6.TNF-/- macrophages and consequences thereof.
Conclusion: Our results indicate that TNF is not required for IFNγ and LPS or LPS alone activation of macrophage phagocytosis. TNF may negatively regulate macrophage phagocytosis of tumour cells.
|Item Type:||Refereed Article|
|Keywords:||Devil facial tumour disease, macrophages, TNF, nitric oxide, phagocytosis|
|Research Division:||Biological Sciences|
|Research Field:||Animal immunology|
|Objective Division:||Environmental Management|
|Objective Group:||Terrestrial systems and management|
|Objective Field:||Terrestrial biodiversity|
|UTAS Author:||Li, X (Ms Amy Li)|
|UTAS Author:||Darby, J (Ms Jocelyn Darby)|
|UTAS Author:||Lyons, AB (Associate Professor Bruce Lyons)|
|UTAS Author:||Woods, GM (Professor Gregory Woods)|
|UTAS Author:||Korner, H (Professor Heinrich Korner)|
|Web of Science® Times Cited:||5|
|Deposited By:||Menzies Institute for Medical Research|
|Downloads:||29 View Download Statistics|
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