eCite Digital Repository

Development of the cellular immune system of Drosophila requires the membrane attack complex/perforin-like protein Torso-like


Forbes-Beadle, L and Crossman, T and Johnson, TK and Burke, R and Warr, C and Whisstock, JC, Development of the cellular immune system of Drosophila requires the membrane attack complex/perforin-like protein Torso-like, Genetics, 204, (2) pp. 675-681. ISSN 0016-6731 (2016) [Refereed Article]

DOI: doi:10.1534/genetics.115.185462


Pore-forming members of the membrane attack complex/perforin-like (MACPF) protein superfamily perform well-characterized roles as mammalian immune effectors. For example, complement component 9 and perforin function to directly form pores in the membrane of Gram-negative pathogens or virally infected/transformed cells, respectively. In contrast, the only known MACPF protein in Drosophila melanogaster, Torso-like, plays crucial roles during development in embryo patterning and larval growth. Here, we report that in addition to these functions, Torso-like plays an important role in Drosophila immunity. However, in contrast to a hypothesized effector function in, for example, elimination of Gram-negative pathogens, we find that torso-like null mutants instead show increased susceptibility to certain Gram-positive pathogens such as Staphylococcus aureus and Enterococcus faecalis. We further show that this deficit is due to a severely reduced number of circulating immune cells and, as a consequence, an impaired ability to phagocytose bacterial particles. Together these data suggest that Torso-like plays an important role in controlling the development of the Drosophila cellular immune system. © 2016 by the Genetics Society of America.

Item Details

Item Type:Refereed Article
Keywords:Cellular immunity; Drosophila melanogaster; Genetics of immunity; Membrane attack complex/perforin-like proteins; Phagocytosis; Torso-like; complement membrane attack complex; Drosophila protein; membrane attack complex perforin like protein; adult
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Developmental genetics (incl. sex determination)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Warr, C (Professor Coral Warr)
ID Code:131331
Year Published:2016
Web of Science® Times Cited:9
Deposited By:Medicine
Deposited On:2019-03-13
Last Modified:2019-03-13

Repository Staff Only: item control page