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A role for the Drosophila zinc transporter Zip88E in protecting against dietary zinc toxicity
Citation
Richards, CD and Warr, C and Burke, R, A role for the Drosophila zinc transporter Zip88E in protecting against dietary zinc toxicity, PLoS ONE, 12, (7) Article e0181237. ISSN 1932-6203 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 Richards et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1371/journal.pone.0181237
Abstract
Zinc absorption in animals is thought to be regulated in a local, cell autonomous manner with intestinal cells responding to dietary zinc content. The Drosophila zinc transporter Zip88E shows strong sequence similarity to Zips 42C.1, 42C.2 and 89B as well as mammalian Zips 1, 2 and 3, suggesting that it may act in concert with the apically-localised Drosophila zinc uptake transporters to facilitate dietary zinc absorption by importing ions into the midgut enterocytes. However, the functional characterisation of Zip88E presented here indicates that Zip88E may instead play a role in detecting and responding to zinc toxicity. Larvae homozygous for a null Zip88E allele are viable yet display heightened sensitivity to elevated levels of dietary zinc. This decreased zinc tolerance is accompanied by an overall decrease in Metallothionein B transcription throughout the larval midgut. A Zip88E reporter gene is expressed only in the salivary glands, a handful of enteroendocrine cells at the boundary between the anterior and middle midgut regions, and in two parallel strips of sensory cell projections connecting to the larval ventral ganglion. Zip88E expression solely in this restricted subset of cells is sufficient to rescue the Zip88E mutant phenotype. Together, our data suggest that Zip88E may be functioning in a small subset of cells to detect excessive zinc levels and induce a systemic response to reduce dietary zinc absorption and hence protect against toxicity.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | unclassified drug, zinc, zinc transporter, Zip88E protein, carrier protein, zinc, zinc-binding protein, adult, animal experiment, animal tissue, arthropod larva |
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Cell metabolism |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Warr, C (Professor Coral Warr) |
| ID Code: | 131314 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 7 |
| Deposited By: | Medicine |
| Deposited On: | 2019-03-13 |
| Last Modified: | 2019-04-26 |
| Downloads: | 26 View Download Statistics |
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