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Spoilage microbial community profiling by 16S rRNA amplicon sequencing of modified atmosphere packaged live mussels stored at 4oC


Odeyemi, OA and Burke, CM and Bolch, CCJ and Stanley, R, Spoilage microbial community profiling by 16S rRNA amplicon sequencing of modified atmosphere packaged live mussels stored at 4oC, Food Research International, 121 pp. 568-576. ISSN 0963-9969 (2018) [Refereed Article]

Copyright Statement

2018 Elsevier Ltd. All rights reserved.

DOI: doi:10.1016/j.foodres.2018.12.017


There is little information on the microbial communities associated with modified atmosphere (MA)-packaged live mussels. There is also a dearth of information on how pre-packaging depuration modifies the microbial communities and spoilage of live mussels. Amplicon sequencing was used to describe spoilage microbial succession in MA-packaged live mussels during storage at 4 C. Proteobacteria, Cyanobacteria and Firmicutes were the three major phyla observed in the mussel meat and pouch water of undepurated and depurated mussels. Among these phyla, Cyanobacteria was more predominant on day 0 in mussel meat of undepurated and depurated mussels while Proteobacteria was predominant in commercially-depurated mussels. Synechococcus was apparently dominant on days 07 in the meat of undepurated mussels and days 010 in depurated mussels. Shewanella was dominant on day 0 in commercially-depurated mussels and dominant on day 15 in undepurated while Acidaminococcus was dominant in depurated mussels on day 15. Psychromonas was observed to be dominant in commercially-depurated mussels on day 7 and further shifted to Acinetobacter by day 10 and 15. In the pouch water, Acinetobacter was dominant throughout the storage days in undepurated mussels while Psychrobacter was predominant in both depurated and commercially-depurated mussels. This study demonstrated the impact of depuration on the microbiota and the spoilage mechanism of MA-packaged live mussels. Shewanella was easily removed through depuration. However, spoilage bacteria such as Acidaminococcus could not be easily removed although they are not important at the beginning but grew at the end. Pouch water contributed suitable biological medium for the growth of Acinetobacter and Psychrobacter and both enhanced the growth of spoilage bacteria such as Shewanella and Acidaminococcus.

Item Details

Item Type:Refereed Article
Keywords:bacteria, spoilage, mussels, modified atmosphere packaging, microbiota, depuration
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture molluscs (excl. oysters)
UTAS Author:Odeyemi, OA (Dr Olumide Odeyemi)
UTAS Author:Burke, CM (Dr Chris Burke)
UTAS Author:Bolch, CCJ (Associate Professor Christopher Bolch)
UTAS Author:Stanley, R (Professor Roger Stanley)
ID Code:131096
Year Published:2018
Web of Science® Times Cited:13
Deposited By:Ecology and Biodiversity
Deposited On:2019-03-01
Last Modified:2022-09-01

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