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130872 - Lactobacillus acidophilus DDS 1 modulates the gut microbiota and improves metabolic profiles in aging mice.pdf (4.83 MB)

Lactobacillus acidophilus DDS-1 modulates the gut microbiota and improves metabolic profiles in aging mice

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posted on 2023-05-20, 00:57 authored by Vemuri, R, Shinde, TS, Gundamaraju, R, Gondalia, SV, Karpe, AV, Beale, DJ, Martoni, CJ, Rajaraman Eri
Recent evidence suggests that gut microbiota shifts can alter host metabolism even during healthy aging. Lactobacillus acidophilus DDS-1, a probiotic strain, has shown promising probiotic character in vitro, as well as in clinical studies. The present study was carried out to investigate whether DDS-1 can modulate the host metabolic phenotype under the condition of age-affected gut microbial shifts in young and aging C57BL/6J mice. Collected fecal samples were analyzed using 16S rRNA gene sequencing for identifying gut microbiota and untargeted gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Gut microbial shifts were observed in the control groups (young and aging), leading to an alteration in metabolism. Principal coordinate analysis (PCoA) of microbiota indicated distinct separation in both the DDS-1-treated groups. L. acidophilus DDS-1 increased the relative abundances of beneficial bacteria, such as Akkermansia muciniphila and Lactobacillus spp., and reduced the relative levels of opportunistic bacteria such as Proteobacteria spp. Metabolic pathway analysis identified 10 key pathways involving amino acid metabolism, protein synthesis and metabolism, carbohydrate metabolism, and butanoate metabolism. These findings suggest that modulation of gut microbiota by DDS-1 results in improvement of metabolic phenotype in the aging mice.

History

Publication title

Nutrients

Volume

10

Issue

9

Article number

1255

Number

1255

Pagination

1-19

ISSN

2072-6643

Department/School

School of Health Sciences

Publisher

MDPI Publishing

Place of publication

Switzerland

Rights statement

Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

Repository Status

  • Open

Socio-economic Objectives

Expanding knowledge in the health sciences

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