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Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples

Citation

See-Too, WS and Ambrose, M and Malley, R and Ee, R and Mulcahy, E and Manche, E and Lazenby, J and McEwan, B and Pagnon, J and Chen, JW and Chan, KG and Turnbull, L and Whitchurch, CB and Roddam, LF, Pandoraea fibrosis sp. nov., a novel Pandoraea species isolated from clinical respiratory samples, International Journal of Systematic and Evolutionary Microbiology, 69, (3) pp. 645-651. ISSN 1466-5026 (2019) [Refereed Article]


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Copyright 2019 IUMS

DOI: doi:10.1099/ijsem.0.003147

Abstract

Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and identified as Pandoraea pnomenusa underwent a polyphasic taxonomic analysis. The isolates were found to be Gram-negative, facultative anaerobic motile bacilli and subsequently designated as strains 6399T (=LMG29626T=DSM103228T) and 7641 (=LMG29627=DSM103229), respectively. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that 6399T and 7641 formed a distinct phylogenetic lineage within the genus Pandoraea. Genome sequence comparison analysis indicated that strains 6399T and 7641 are clonal and share 100 % similarity, however, similarity to other type strains (ANIb 73.2-88.8 %, ANIm 83.5-89.9 % and OrthoANI 83.2-89.3 %) indicates that 6399T and 7641 do not belong to any of the reported type species. The major cellular fatty acids of 6399T were C16 : 0 (32.1 %) C17 : 0cyclo (18.7 %) and C18 : 7c (14.5 %), while Q-8 was the only respiratory quinone detected. The major polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of 6399T was 62.9 (mol%). Strain 6399T can be differentiated from other members of Pandoraea by the absence of C19 : 0ω8c cyclo and by the presence of C17 : 8c cyclo. Together our data show that the bacterial strains 6399T and 7641 represent a novel species of the genus Pandoraea, for which the name Pandoraea fibrosis sp. nov. is proposed (type strain 6399T).

Item Details

Item Type:Refereed Article
Keywords:Pandoraea; cystic fibrosis
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
UTAS Author:Ambrose, M (Dr Mark Ambrose)
UTAS Author:Malley, R (Dr Roslyn Malley)
UTAS Author:Mulcahy, E (Miss Emily Mulcahy)
UTAS Author:Manche, E (Ms Emily Manche)
UTAS Author:Pagnon, J (Ms Joanne Pagnon)
UTAS Author:Roddam, LF (Dr Louise Roddam)
ID Code:130826
Year Published:2019
Deposited By:Medicine
Deposited On:2019-02-14
Last Modified:2019-06-17
Downloads:0

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