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Membrane-free electrokinetic device integrated to electrospray-ionization mass spectrometry for the simultaneous removal of sodium dodecyl sulfate and enrichment of peptides

Citation

Tubaon, RM and Haddad, PR and Quirino, JP, Membrane-free electrokinetic device integrated to electrospray-ionization mass spectrometry for the simultaneous removal of sodium dodecyl sulfate and enrichment of peptides, Analytical Chemistry, 90, (17) pp. 10122-10127. ISSN 0003-2700 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 American Chemical Society

DOI: doi:10.1021/acs.analchem.8b01707

Abstract

The removal of sodium dodecyl sulfate (SDS) in SDS-assisted proteomics with electrospray-ionization-mass-spectrometric (ESI-MS) analysis is an essential step in the analysis. Off-line state-of-the-art sample-preparation strategies can allow 100% removal of DS- and up to 100% peptide recoveries. These strategies, however, are typically laborious and require long analysis times and a complex experimental setup. Here, we developed a simple, membrane-free, electrokinetic, on-line, integrated SDS removal–ESI-MS device that was able to enhance ESI-MS signals of bradykinin and peptides from trypsin-digested bovine serum albumin (BSA) in samples that contained SDS micelles. The significant peptide-signal improvements were contributed by the complete removal of DS- and the enrichment of the peptides in the presence of an electric field. Enrichment was via micelle-to-solvent stacking, initially developed in capillary electrophoresis. Bradykinin percent recovery was 800%, and BSA peptide percent recovery was 87%. Enhancement factors in ESI-MS signals (after and before removal) for selected m/z values of peptides from the BSA digest were 535-693.

Item Details

Item Type:Refereed Article
Keywords:mass spectrometry, sample preparation
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
UTAS Author:Tubaon, RM (Ms Ria Tubaon)
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:130522
Year Published:2018
Funding Support:Australian Research Council (DP180102810)
Deposited By:Chemistry
Deposited On:2019-01-30
Last Modified:2019-03-07
Downloads:0

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