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High performance liquid chromatography as a molecular probe in quantitative structure-retention relationships studies of selected lipid classes on polar-embedded stationary phases

Citation

Buszewski, B and Walczak, J and Skoczylas, M and Haddad, PR, High performance liquid chromatography as a molecular probe in quantitative structure-retention relationships studies of selected lipid classes on polar-embedded stationary phases, Journal of Chromatography A, 1585 pp. 105-112. ISSN 0021-9673 (2019) [Refereed Article]

Copyright Statement

2018 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.chroma.2018.11.053

Abstract

Studies on the retention mechanism of lipid classes (phospholipids, sphingomyelin) were performed using three polar-embedded stationary phases for which diol, phosphate, amino, and amide moieties were incorporated into the alkyl chains of the stationary phases. Their structural descriptors were determined using the quantum-mechanical method. The retention behavior of the analytes was investigated as a function of different binary hydro-organic mobile phases containing (90%/10% acetonitrile (or methanol)/0.1% formic acid). It was found that the elution order on the tested stationary phases was governed chiefly by the hydrophilicity of the analyte and indicated the existence of a hydrophilic interaction liquid chromatography retention mechanism. Quantitative structure-retention relationships studies were performed to further elucidate the retention mechanism. These studies showed that the dominant analyte descriptor influencing retention on the alkyl-amine stationary phase was the logarithm of the octanol-water partition coefficient. For the phospho-diol and alkyl-amide stationary phases, the dominant analyte descriptor influencing retention was the molar volume and solvent accessible area of the analyte, respectively.

Item Details

Item Type:Refereed Article
Keywords:lipids, phospholipids, polar-embedded stationary phase, QSRR, retention mechanism
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:130493
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2019-01-29
Last Modified:2019-03-25
Downloads:0

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