130225 - Single-cell profiling identifies key pathways.pdf (12.06 MB)
Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media
journal contribution
posted on 2023-05-19, 23:56 authored by Daniszewski, M, Nguyen, Q, Chy, HS, Vikrant Singh, Crombie, DE, Kulkarni, T, Liang, HH, Sivakumaran, P, Lidgerwood, GE, Hernandez, D, Conquest, A, Rooney, LA, Chevalier, S, Andersen, SB, Senabouth, A, James VickersJames Vickers, Mackey, DA, Craig, JE, Laslett, AL, Alexander HewittAlexander Hewitt, Powell, JE, Pebay, AWe assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.
History
Publication title
iScienceVolume
7Pagination
30-39ISSN
2589-0042Department/School
Menzies Institute for Medical ResearchPublisher
Cell PressPlace of publication
United StatesRights statement
Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open