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Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media

Citation

Daniszewski, M and Nguyen, Q and Chy, HS and Singh, V and Crombie, DE and Kulkarni, T and Liang, HH and Sivakumaran, P and Lidgerwood, GE and Hernandez, D and Conquest, A and Rooney, LA and Chevalier, S and Andersen, SB and Senabouth, A and Vickers, JC and Mackey, DA and Craig, JE and Laslett, AL and Hewitt, AW and Powell, JE and Pebay, A, Single-cell profiling identifies key pathways expressed by iPSCs cultured in different commercial media, iScience, 7 pp. 30-39. ISSN 2589-0042 (2018) [Refereed Article]


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DOI: doi:10.1016/j.isci.2018.08.016

Abstract

We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.

Item Details

Item Type:Refereed Article
Keywords:automation, stem cells research, transcriptomics
Research Division:Medical and Health Sciences
Research Group:Ophthalmology and Optometry
Research Field:Ophthalmology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Hearing, Vision, Speech and Their Disorders
UTAS Author:Singh, V (Mr Vikrant Singh)
UTAS Author:Vickers, JC (Professor James Vickers)
UTAS Author:Hewitt, AW (Professor Alex Hewitt)
ID Code:130225
Year Published:2018
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-01-16
Last Modified:2019-01-16
Downloads:0

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