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The loss of RBOHD function modulates root adaptive responses to combined hypoxia and salinity stress in Arabidopsis

Citation

Wang, F and Chen, Z-H and Lui, X and Shabala, L and Yu, M and Zhou, M and Salih, A and Shabala, S, The loss of RBOHD function modulates root adaptive responses to combined hypoxia and salinity stress in Arabidopsis, Environmental and Experimental Botany, 158 pp. 125-135. ISSN 0098-8472 (2019) [Refereed Article]

Copyright Statement

Copyright 2018 Elsevier B.V.

DOI: doi:10.1016/j.envexpbot.2018.11.020

Abstract

Salinity stress in nature is often accompanied by soil waterlogging. The constraints imposed by this additional stress have a profound effect on Na+ and Cl transport from roots to shoots, thus affecting homeostasis of some essential ions such as K+ or Ca2+ and influencing plant growth. The underlying mechanisms, however, remain largely unknown. In this study, we used a range of electrophysiological (ion flux measuring MIFE) and imaging (fluorescence dyes) techniques to investigate the role of Respiratory Burst Oxidase Homolog protein D (RBOHD) in Arabidopsis root responses to combined salinity and hypoxia stress. We found that combined stress causes more damage to plants than salinity stress alone, and the rbohD mutant is more sensitive to both treatments compared with wild type (WT). Mild hypoxia stress (root exposure to N2-bullbed solution for 48 h) reduced detrimental impact of salinity on the magnitude of NaCl-induced K+ loss from the root in wild type; this effect, however, was not observed in rbohD mutant. In salt-treated plants, onset of hypoxia led to increased uptake of Na+ and Cl in plants lacking functional RBOHD protein but not in a wild type. The rbohD mutant lacked ability for stress-induced H2O2 production and accumulated more Ca2+ and Na+ than WT under both salinity and combined stress. These results suggested RBOHD plays an important role in the regulation of downstream Ca2+ signal and H2O2 production, thus affecting plant ionic homeostasis, and that the lack of functional RBOHD proteins compromises plant ability to minimise Na+ accumulation under salinity and combined stress.

Item Details

Item Type:Refereed Article
Keywords:root epidermis, K+ flux, reactive oxygen species, stele, hydrogen peroxide, cellular signalling, hypoxia, salinity, calcium, acclimation
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
UTAS Author:Wang, F (Miss Feifei Wang)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:130054
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Agriculture and Food Systems
Deposited On:2019-01-08
Last Modified:2019-03-12
Downloads:0

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