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Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi

Citation

Zeng, F and Shabala, S and Maksimovic, JD and Maksimovic, V and Bonales-Alatorre, E and Shabala, L and Yu, M and Zhang, G and Zvanovic, BD, Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi, Plant Cell and Environment, 41, (11) pp. 2654-2667. ISSN 0140-7791 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 John Wiley & Sons Ltd.

DOI: doi:10.1111/pce.13391

Abstract

Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of Crossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+‐ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch‐clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.

Item Details

Item Type:Refereed Article
Keywords:vacuolar sodium sequestration, tonoplast channels, SV, FV, potassium retention, reactive oxygen species, antioxidant activity, mesophyll, storage parenchyma, potassium, sodium, salinity, ROS, vacuole
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
UTAS Author:Zeng, F (Dr Fanrong Zeng)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Bonales-Alatorre, E (Dr Edgar Bonales Alatorre)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
ID Code:130053
Year Published:2018
Web of Science® Times Cited:4
Deposited By:Agriculture and Food Systems
Deposited On:2019-01-08
Last Modified:2019-03-08
Downloads:0

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