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Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi
journal contribution
posted on 2023-05-19, 23:42 authored by Zeng, F, Sergey ShabalaSergey Shabala, Maksimovic, JD, Maksimovic, V, Bonales-Alatorre, E, Svetlana ShabalaSvetlana Shabala, Yu, M, Zhang, G, Zvanovic, BDEfforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii (“pigface”). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+‐ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch‐clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.
History
Publication title
Plant Cell and EnvironmentVolume
41Issue
11Pagination
2654-2667ISSN
0140-7791Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Blackwell Publishing LtdPlace of publication
9600 Garsington Rd, Oxford, England, Oxon, Ox4 2DgRights statement
Copyright 2018 John Wiley & Sons Ltd.Repository Status
- Restricted