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qPCR assays for the detection and quantification of multiple paralytic shellfish toxin-producing species of Alexandrium


Ruvindy, R and Bolch, CJ and MacKenzie, L and Smith, KF and Murray, SA, qPCR assays for the detection and quantification of multiple paralytic shellfish toxin-producing species of Alexandrium, Frontiers in Microbiology, 9 Article 3153. ISSN 1664-302X (2018) [Refereed Article]


Copyright Statement

Copyright 2018 Ruvindy, Bolch, MacKenzie, Smith and Murray. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.3389/fmicb.2018.03153


Paralytic shellfish toxin producing dinoflagellates have negatively impacted the shellfish aquaculture industry worldwide, including in Australia and New Zealand. Morphologically identical cryptic species of dinoflagellates that may differ in toxicity, in particular, species of the former Alexandrium tamarense species complex, co-occur in Australia, as they do in multiple regions in Asia and Europe. To understand the dynamics and the ecological drivers of the growth of each species in the field, accurate quantification at the species level is crucial. We have developed the first quantitative polymerase chain reaction (qPCR) primers for A. australiense, and new primers targeting A. ostenfeldii, A. catenella, and A. pacificum. We showed that our new primers for A. pacificum are more specific than previously published primer pairs. These assays can be used to quantify planktonic cells and cysts in the water column and in sediment samples with limits of detection of 2 cells/L for the A. catenella and A. australiense assays, 2 cells/L and 1 cyst/mg sediment for the A. pacificum assay, and 1 cells/L for the A. ostenfeldii assay, and efficiencies of >90%. We utilized these assays to discriminate and quantify co-occurring A. catenella, A. pacificum, and A. australiense in samples from the east coast of Tasmania, Australia.

Item Details

Item Type:Refereed Article
Keywords:Alexandrium, toxic dinoflagellate, Australia, qPCR detection, qPCR, ribosomal DNA, dinoflagellate cysts
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Phycology (incl. marine grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture oysters
UTAS Author:Bolch, CJ (Associate Professor Christopher Bolch)
ID Code:129907
Year Published:2018
Web of Science® Times Cited:21
Deposited By:Ecology and Biodiversity
Deposited On:2018-12-20
Last Modified:2019-03-12
Downloads:583 View Download Statistics

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