Yen, S and Preissner, M and Bennett, E and Dubsky, S and Carnibella, R and O'Toole, R and Roddam, L and Jones, H and Dargaville, PA and Fouras, A and Zosky, GR, The link between regional tidal stretch and lung injury during mechanical ventilation, American Journal of Respiratory Cell and Molecular Biology pp. 1-35. ISSN 1535-4989 (2018) [Refereed Article]
Copyright © 2019 American Thoracic Society
Methods: Two groups of BALB/c mice (n = 8 per group) were ventilated for 2 h using a protective or injurious ventilation strategy, with free-breathing mice as controls. Regional VT and FRC of the ventilated mice was determined from analysis of high-resolution four-dimensional computed tomography (4D-CT) images, taken at baseline and after 2 h of ventilation and corrected for the volume of the region (i.e. specific [s]Vt and specific [s]FRC). RNA levels of 21 genes in ten different lung regions were quantified using qPCR array.
Results: sFRC at baseline varied regionally, independent of ventilation strategy, whereas sVT varied regionally depending on ventilation strategy. The expression of IL-6 (P = 0.04), Ccl2 (P < 0.01) and Ang 2 (P < 0.05) were associated with sVT but not sFRC. The expression of seven other genes varied regionally (IL-1β and RAGE) or depended on ventilation strategy (Nfe2l2, c-fos and Wnt1) or both (TNF-α and CxCl2), but were not associated with regional sFRC or sVT.
Conclusion: These observations suggest that regional inflammatory responses to mechanical ventilation are primarily driven by tidal stretch.
|Item Type:||Refereed Article|
|Keywords:||tidal stretch, lung injury, mechanical ventilation|
|Research Division:||Medical and Health Sciences|
|Research Group:||Cardiorespiratory Medicine and Haematology|
|Research Field:||Respiratory Diseases|
|Objective Group:||Clinical Health (Organs, Diseases and Abnormal Conditions)|
|Objective Field:||Respiratory System and Diseases (incl. Asthma)|
|UTAS Author:||Yen, S (Mr Seiha Yen)|
|UTAS Author:||Bennett, E (Ms Ellen Bennett)|
|UTAS Author:||O'Toole, R (Dr Ronan O'Toole)|
|UTAS Author:||Roddam, L (Dr Louise Roddam)|
|UTAS Author:||Dargaville, PA (Professor Peter Dargaville)|
|UTAS Author:||Zosky, GR (Professor Graeme Zosky)|
|Web of Science® Times Cited:||1|
|Deposited By:||Menzies Institute for Medical Research|
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