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Proteomics reveals ablation of PlGF increases antioxidant and neuroprotective proteins in the diabetic mouse retina
Citation
Saddala, MS and Lennikov, A and Grab, DJ and Liu, G-S and Tang, S and Huang, H, Proteomics reveals ablation of PlGF increases antioxidant and neuroprotective proteins in the diabetic mouse retina, Scientific Reports, 8, (1) Article 16728. ISSN 2045-2322 (2018) [Refereed Article]
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Copyright Statement
Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1038/s41598-018-34955-x
Abstract
Placental growth factor (PlGF or PGF), a member of the vascular endothelial growth factor (VEGF) sub-family, plays a crucial role in pathological angiogenesis and inflammation. However, the underlying molecular mechanisms that PlGF mediates regarding the complications of non-proliferative diabetic retinopathy (DR) remain elusive. Using an LC-MS/MS-based label-free quantification proteomic approach we characterized the alterations in protein expression caused by PlGF ablation in the retinas obtained from C57BL6, Akita, PlGF-/- and Akita.PlGF-/- mice. After extraction and enzymatic digestion with Trypsin/LysC, the retinal proteins were analyzed by Q-Exactive hybrid Quadrupole-Orbitrap mass spectrometry. Differentially expressed proteins (DEPs) were identified in four comparisons based on Z-score normalization and reproducibility by Pearson's correlation coefficient. The gene ontology (GO), functional pathways, and protein-protein network interaction analysis suggested that several proteins involved in insulin resistance pathways (Gnb1, Gnb2, Gnb4, Gnai2, Gnao1, Snap2, and Gngt1) were significantly down-regulated in PlGF ablated Akita diabetic mice (Akita.PlGF-/- vs. Akita) but up-regulated in Akita vs. C57 and PlGF-/- vs. C57 conditions. Two proteins involved in the antioxidant activity and neural protection pathways, Prdx6 and Map2 respectively, were up-regulated in the Akita.PlGF-/- vs. Akita condition. Overall, we predict that down-regulation of proteins essential for insulin resistance, together with the up-regulation of antioxidant and neuroprotection proteins highlight and epitomize the potential mechanisms important for future anti-PlGF therapies in the treatment of DR.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | diabetic retinopathy, placenta growth factor, proteomic |
| Research Division: | Medical and Health Sciences |
| Research Group: | Ophthalmology and Optometry |
| Research Field: | Ophthalmology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Medical and Health Sciences |
| UTAS Author: | Liu, G-S (Dr Guei-Sheung Liu) |
| ID Code: | 129830 |
| Year Published: | 2018 |
| Web of Science® Times Cited: | 4 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2018-12-19 |
| Last Modified: | 2019-03-15 |
| Downloads: | 66 View Download Statistics |
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