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Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue


Ding, Y and Chow, SH and Liu, G-S and Wang, B and Lin, T-W and Hsu, H-Y and Duff, AP and Le Brun, AP and Shen, H-H, Annexin V-containing cubosomes for targeted early detection of apoptosis in degenerative retinal tissue, Journal of Materials Chemistry B, 6, (46) pp. 7652-7661. ISSN 2050-750X (2018) [Refereed Article]

Copyright Statement

© The Royal Society of Chemistry 2018

DOI: doi:10.1039/c8tb02465k


New drug delivery materials targeting damaged ocular tissues are of particular interest. In this work, we have formulated annexin/phosphatidylserine/phytantriol and annexin/phosphatidylserine/monoolein cubosomes based on incorporation of 1,2-dipalmitoyl-sn-glycero-3-phospho-L-serine (PS) lipid and annexin V (ANX) protein with phytantriol (Phy) and monoolein (MO) respectively. The incorporation of ANX is important because it can be used as a diagnostic tool for in vivo apoptosis detection due to its high affinity to phosphatidylserine in the presence of Ca2+. We have also prepared PS–Phy and PS–MO cubosomes without ANX as a comparison, and characterized them using dynamic light scattering, cryo-TEM images and small-angle X-ray scattering, showing that PS–Phy cubosomes have greater chemical stability, and that ANX–PS–Phy cubosomes have the potential for in vivo drug delivery. In addition, we have reconstituted an apoptotic biomimetic membrane on a surface to gain insights into cubosome–bilayer interactions using a quartz-crystal microbalance and neutron reflectometry. The neutron reflectivity data reveal that there is exchange of materials between the biomimetic apoptotic bilayer and ANX–PS–Phy cubosomes, with an accumulation of ANX between the membrane and cubosomes possibly being the reason for the reduced cytotoxicity of ANX–PS–Phy cubosomes. A rat model of laser-induced choroidal neovascularization showed that ANX–PS–Phy cubosomes specifically targeted apoptotic cells in vivo. We propose that ANX–PS–Phy cubosomes are a potential candidate for ocular drug delivery for eye diseases.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biotechnology
Research Field:Gene and molecular therapy
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Liu, G-S (Associate Professor Guei-Sheung Liu)
ID Code:129826
Year Published:2018
Web of Science® Times Cited:8
Deposited By:Menzies Institute for Medical Research
Deposited On:2018-12-19
Last Modified:2022-08-26

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