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The HDAC6 Inhibitor Trichostatin A Acetylates Microtubules and Protects Axons From Excitotoxin-Induced Degeneration in a Compartmented Culture Model

Citation

Hanson, K and Tian, N and Vickers, JC and King, AE, The HDAC6 Inhibitor Trichostatin A Acetylates Microtubules and Protects Axons From Excitotoxin-Induced Degeneration in a Compartmented Culture Model, Frontiers in Neuroscience, 12 Article 872. ISSN 1662-453X (2018) [Refereed Article]


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Copyright Statement

Copyright 2018 Hanson, Tian, Vickers and King. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fnins.2018.00872

Abstract

Axon degeneration has been implicated as a pathological process in several neurodegenerative diseases and acquired forms of neural injury. We have previously shown that stabilizing microtubules can protect axons against excitotoxin-induced fragmentation, however, the alterations of microtubules following excitotoxicity that results in axon degeneration are currently unknown. Hence, this study investigated whether excitotoxicity affects the post-translational modifications of microtubules and microtubule-associated proteins, and whether reversing these changes has the potential to rescue axons from degeneration. To investigate microtubule alterations, primary mouse cortical neurons at 10 days in vitro were treated with 10 or 25 μM kainic acid to induce excitotoxicity and axon degeneration. Post-translational modifications of microtubules and associated proteins were examined at 6 h following kainic acid exposure, relative to axon degeneration. While there were no changes to tyrosinated tubulin or MAP1B, acetylated tubulin was significantly (p < 0.05) decreased by 40% at 6 h post-treatment. To determine whether increasing microtubule acetylation prior to kainic acid exposure could prevent axon fragmentation, we investigated the effect of reducing microtubule deacetylation with the HDAC6 inhibitor, trichostatin A. We found that trichostatin A prevented kainic acid-induced microtubule deacetylation and significantly (p < 0.05) protected axons from fragmentation. These data suggest that microtubule acetylation is a potential target for axonal protection where excitotoxicity may play a role in neuronal degeneration.

Item Details

Item Type:Refereed Article
Keywords:Dementia, Motor Neuron Disease, Excitotoxicity, Neuron, Axon, Microtubule, Acetylated microtubule
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Cellular Nervous System
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Neurodegenerative Disorders Related to Ageing
UTAS Author:Hanson, K (Miss Kelsey Hanson)
UTAS Author:Tian, N (Dr Nan Tian)
UTAS Author:Vickers, JC (Professor James Vickers)
UTAS Author:King, AE (Associate Professor Anna King)
ID Code:129685
Year Published:2018
Funding Support:National Health and Medical Research Council (1085221)
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2018-12-13
Last Modified:2019-01-04
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