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Combined cytotoxicity of the phycotoxin okadaic acid and mycotoxins on intestinal and neuroblastoma human cell models

Citation

Hayashi, A and Dorantes-Aranda, JJ and Bowman, JP and Hallegraeff, G, Combined cytotoxicity of the phycotoxin okadaic acid and mycotoxins on intestinal and neuroblastoma human cell models, Toxins, 10, (12) Article 526. ISSN 2072-6651 (2018) [Refereed Article]


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Copyright 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/toxins10120526

Abstract

Mycotoxins are emerging toxins in the marine environment, which can co-occur with algal toxins to exert synergistic or antagonistic effects for human seafood consumption. The current study assesses the cytotoxicity of the algal toxin okadaic acid, shellfish, and dust storm-associated mycotoxins alone or in combination on human intestinal (HT-29) and neuroblastoma (SH-SY5Y) cell lines. Based on calculated IC50 (inhibitory concentration 50%) values, mycotoxins and the algal toxin on their own exhibited increased cytotoxicity in the order of sydowinin A < sydowinin B << patulin < alamethicin < sydowinol << gliotoxin = okadaic acid against the HT-29 cell line, and sydowinin B < sydowinin A << alamethicin = sydowinol < patulin, << gliotoxin < okadaic acid against the SH-SY5Y cell line. Combinations of okadaic acid–sydowinin A, –alamethicin, –patulin, and –gliotoxin exhibited antagonistic effects at low-moderate cytotoxicity, but became synergistic at high cytotoxicity, while okadaic acid–sydowinol displayed an antagonistic relationship against HT-29 cells. Furthermore, only okadaic acid–sydowinin A showed synergism, while okadaic acid–sydowinol, –alamethicin, –patulin, and –gliotoxin combinations demonstrated antagonism against SH-SY5Y. While diarrhetic shellfish poisoning (DSP) from okadaic acid and analogues in many parts of the world is considered to be a comparatively minor seafood toxin syndrome, our human cell model studies suggest that synergisms with certain mycotoxins may aggravate human health impacts, depending on the concentrations. These findings highlight the issues of the shortcomings of current regulatory approaches, which do not regulate for mycotoxins in shellfish and treat seafood toxins as if they occur as single toxins.

Item Details

Item Type:Refereed Article
Keywords:okadaic acid, sydowinin A, sydowinol, alamethicin, patulin, gliotoxin, combination index, synergy
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Oysters
UTAS Author:Hayashi, A (Ms Aiko Hayashi)
UTAS Author:Dorantes-Aranda, JJ (Dr Juan Dorantes Aranda)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
UTAS Author:Hallegraeff, G (Professor Gustaaf Hallegraeff)
ID Code:129592
Year Published:2018
Funding Support:Australian Research Council (DP130102725)
Deposited By:Ecology and Biodiversity
Deposited On:2018-12-09
Last Modified:2019-01-04
Downloads:0

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