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Employing pressurized hot water extraction (PHWE) to explore natural products chemistry in the undergraduate laboratory

Citation

Ho, CC and Deans, BJ and Just, J and Warr, GG and Wilkinson, S and Smith, JA and Bissember, AC, Employing pressurized hot water extraction (PHWE) to explore natural products chemistry in the undergraduate laboratory, Journal of Visualized Experiments, 141 Article e58195. ISSN 1940-087X (2018) [Refereed Article]


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Copyright 2018 Journal of Visualized Experiments

DOI: doi:10.3791/58195

Abstract

A recently developed pressurized hot water extraction (PHWE) method which utilizes an unmodified household espresso machine to facilitate natural products research has also found applications as an effective teaching tool. Specifically, this technique has been used to introduce second- and third-year undergraduates to aspects of natural products chemistry in the laboratory. In this report, two experiments are presented: the PHWE of eugenol and acetyleugenol from cloves and the PHWE of seselin and (+)-epoxysuberosin from the endemic Australian plant species Correa reflexa. By employing PHWE in these experiments, the crude clove extract, enriched in eugenol and acetyleugenol, was obtained in 4-9% w/w from cloves by second-year undergraduates and seselin and (+)-epoxysuberosin were isolated in yields of up to 1.1% w/w and 0.9% w/w from C. reflexa by third-year students. The former exercise was developed as a replacement for the traditional steam distillation experiment providing an introduction to extraction and separation techniques, while the latter activity featured guided-inquiry teaching methods in an effort to simulate natural products bioprospecting. This primarily derives from the rapid nature of this PHWE technique relative to traditional extraction methods that are often incompatible with the time constraints associated with undergraduate laboratory experiments. This rapid and practical PHWE method can be used to efficiently isolate various classes of organic molecules from a range of plant species. The complementary nature of this technique relative to more traditional methods has also been demonstrated previously.

Item Details

Item Type:Refereed Article
Keywords:chemistry, chemical education, pressurized hot water extraction, natural products, undergraduate laboratory, bioprospecting, organic chemistry
Research Division:Chemical Sciences
Research Group:Organic Chemistry
Research Field:Natural Products Chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
UTAS Author:Ho, CC (Dr Curtis Ho)
UTAS Author:Deans, BJ (Miss Bianca Deans)
UTAS Author:Just, J (Mr Jeremy Just)
UTAS Author:Smith, JA (Associate Professor Jason Smith)
UTAS Author:Bissember, AC (Dr Alex Bissember)
ID Code:129416
Year Published:2018
Deposited By:Chemistry
Deposited On:2018-11-29
Last Modified:2019-03-06
Downloads:0

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