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Reversed-phase functionalised multi-lumen capillary as combined concentrator, separation column, and ESI emitter in capillary-LC-MS
Citation
Sanz Rodriguez, E and Lam, SC and Haddad, PR and Paull, B, Reversed-phase functionalised multi-lumen capillary as combined concentrator, separation column, and ESI emitter in capillary-LC-MS, Chromatographia, 83, (1) pp. 197-209. ISSN 0009-5893 (2019) [Refereed Article]
Copyright Statement
Copyright 2018 Springer-Verlag GmbH Germany, part of Springer Nature
DOI: doi:10.1007/s10337-018-3629-7
Abstract
For the first time, a multi-lumen capillary (MLC) (126 parallel channels of 4.2 µm i.d) has been modified to produce a C18-functionalised silica porous layer open tubular (PLOT) capillary column for both on-capillary preconcentration and separation. The modified multi-lumen capillary used in this dual mode provided significant advantages over typical nano/capillary-LC–MS systems, in that it facilitated both higher sample loading capacity, the use of elevated flow rates, and simplified equipment requirements. Following modification, 100% of the channels displayed a homogenous porous silica layer, 257 ± 36 nm thick. The PLOT-MLC was first evaluated for on-capillary solid-phase extraction. Extraction of caffeine, ofloxacin, atrazine, and diuron was carried out offline using an 8-cm-long PLOT-MLC, with quantification achieved using HPLC coupled to a quadrupole-time of flight (QTOF) mass spectrometer. The results confirmed reversed-phase selectivity and average recoveries obtained were around 70%. Subsequently, a 65-cm-long PLOT-MLC was evaluated as a separator column using a capillary liquid chromatography (Cap-LC) system equipped with a nano-injector and coupled to the mass spectrometer. The short PLOT-MLC provided a baseline separation in isocratic mode [water:acetonitrile (each with 0.1% formic acid) = 70:30, v/v] of ofloxacin, atrazine, and diuron. Finally, direct coupling of the PLOT-MLC with the QTOF via a capillary electrosprayer facilitated the simultaneous use of the modified capillary as a solid-phase concentrator, separator column (carrying out concentration-focusing-separation on the PLOT-MLC) and electrospray emitter. This configuration greatly simplifies the traditional capillary-LC–MS equipment requirements, via the removal of all connectors and additional capillary between injector and MS inlet, and is demonstrated herein with large volume sample loading and step-gradient elution/separation with sensitive MS detection.
Item Details
Item Type: | Refereed Article |
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Keywords: | liquid chromatography, mass spectrometry, multi-lumen capillary, C18-funtionalised fused silica, porous layer open tubular columns |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez) |
UTAS Author: | Lam, SC (Mr Shing Chung Lam) |
UTAS Author: | Haddad, PR (Professor Paul Haddad) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 129223 |
Year Published: | 2019 (online first 2018) |
Funding Support: | Australian Research Council (IC140100022) |
Web of Science® Times Cited: | 2 |
Deposited By: | Chemistry |
Deposited On: | 2018-11-16 |
Last Modified: | 2019-03-12 |
Downloads: | 0 |
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