eCite Digital Repository

A transcriptomic investigation of appetite-regulation and digestive processes in giant grouper Epinephelus lanceolatus during early larval development

Citation

Anderson, KC and Knuckey, R and Canepa, M and Elizur, A, A transcriptomic investigation of appetite-regulation and digestive processes in giant grouper Epinephelus lanceolatus during early larval development, Journal of Fish Biology, 93, (4) pp. 694-710. ISSN 0022-1112 (2018) [Refereed Article]

Copyright Statement

2018 The Fisheries Society of the British Isles

DOI: doi:10.1111/jfb.13798

Abstract

The giant grouper Epinephelus lanceolatus is an ecologically vulnerable species with high market demand. However, efforts to improve larval husbandry are hindered by a lack of knowledge surrounding larval developmental physiology. To address this shortfall, a transcriptomic approach was applied to larvae between 1 and 14 days post hatch (dph) to characterise the molecular ontogenesis of genes that influence appetite and digestion. Appetite regulating factors were detected from 1 dph, including neuropeptide Y, nesfatin‐1, cocaine and amphetamine regulated transcript, cholecystokinin and pituitary adenylate cyclase activating peptide and the expression level of several genes changed sharply with the onset of exogenous feeding. The level of expression for proteases, chitinases, lipases and amylases typically followed one of two expression patterns, a general increase as development progressed, or an inverted U‐shape with maximal expression at c. 6 dph. Similarly, the tendency among both expression patterns was for the level of expression to increase around the time of mouth‐opening. There was also evidence to suggest the presence of putative isoforms for several digestion‐related genes. We have provided an insight into appetite‐regulation and digestive processes in groupers during early larval development and have developed a transcriptomic database that will aid future efforts to rear this species in an aquaculture setting.

Item Details

Item Type:Refereed Article
Keywords:grouper, larvae, development, gene expression, next-generation sequencing, digestion, ontogenesis
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
UTAS Author:Anderson, KC (Dr Kelli Anderson)
ID Code:128936
Year Published:2018
Web of Science® Times Cited:1
Deposited By:Fisheries and Aquaculture
Deposited On:2018-10-26
Last Modified:2018-11-28
Downloads:0

Repository Staff Only: item control page