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A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells

Citation

Rajendra, KC and Zosky, GR and Shukla, SD and O'Toole, RF, A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells, Biology Methods and Protocols, 3, (1) pp. 1-9. ISSN 2396-8923 (2018) [Refereed Article]


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Copyright Statement

Copyright The Author(s) 2018. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

DOI: doi:10.1093/biomethods/bpy010

Abstract

Nearly half of the world’s population uses biomass fuel for the purposes of cooking and heating. Smoke derived from biomass increases the risk of the development of lung diseases, including pneumonia, chronic obstructive pulmonary disease, airway tract infections, and lung cancer. Despite the evidence linking biomass smoke exposure to pulmonary disease, only a small number of experimental studies have been conducted on the impact of biomass smoke on airway epithelial cells. This is in part due to the lack of a standard and easily accessible procedure for the preparation of biomass smoke. Here, we describe a cost-effective and reproducible method for the generation of different smoke extracts, in particular, cow dung smoke extract (CDSE) and wood smoke extract (WSE) for use in a range of biological applications. We examined the effect of the biomass smoke extracts on human bronchial epithelial cell expression of a known responder to cigarette smoke exposure (CSE), the platelet-activating factor receptor (PAFR). Similar to the treatment with CSE, we observed a dose-dependent increase in PAFR expression on human airway epithelial cells that were exposed to CDSE and WSE. This method provides biomass smoke in a re-usable form for cell and molecular bioscience studies on the pathogenesis of chronic lung disease.

Item Details

Item Type:Refereed Article
Keywords:biomass smoke, cigarette smoke extract, chronic obstructive pulmonary disease, platelet-activating factor receptor, non-typeable haemophilus influenzae
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Rajendra, KC (Mr Rajendra KC)
Author:Zosky, GR (Associate Professor Graeme Zosky)
Author:O'Toole, RF (Dr Ronan O'Toole)
ID Code:128685
Year Published:2018
Deposited By:Medicine (Discipline)
Deposited On:2018-10-06
Last Modified:2018-11-07
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