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Altered expression of the malate-permeable anion channel OsALMT4 reduces the growth of rice under low radiance

Citation

Liu, J and Xu, M and Estavillo, GM and Delhaize, E and White, RG and Zhou, M and Ryan, PR, Altered expression of the malate-permeable anion channel OsALMT4 reduces the growth of rice under low radiance, Frontiers in Plant Science, 9 Article 542. ISSN 1664-462X (2018) [Refereed Article]


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Copyright Statement

Copyright © 2018 Liu, Xu, Estavillo, Delhaize, White, Zhou and Ryan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

DOI: doi:10.3389/fpls.2018.00542

Abstract

We examined the function of OsALMT4 in rice (Oryza sativa L.) which is a member of the aluminum-activated malate transporter family. Previous studies showed that OsALMT4 localizes to the plasma membrane and that expression in transgenic rice lines results in a constitutive release of malate from the roots. Here, we show that OsALMT4 is expressed widely in roots, shoots, flowers, and grain but not guard cells. Expression was also affected by ionic and osmotic stress, light and to the hormones ABA, IAA, and salicylic acid. Malate efflux from the transgenic plants over-expressing OsALMT4 was inhibited by niflumate and salicylic acid. Growth of transgenic lines with either increased OsALMT4 expression or reduced expression was measured in different environments. Light intensity caused significant differences in growth between the transgenic lines and controls. When day-time light was reduced from 700 to 300 μmol m-2s-1 independent transgenic lines with either increased or decreased OsALMT4 expression accumulated less biomass compared to their null controls. This response was not associated with differences in photosynthetic capacity, stomatal conductance or sugar concentrations in tissues. We propose that by disrupting malate fluxes across the plasma membrane carbon partitioning and perhaps signaling are affected which compromises growth under low light. We conclude that OsALMT4 is expressed widely in rice and facilitates malate efflux from different cell types. Altering OsALMT4 expression compromises growth in low-light environments.

Item Details

Item Type:Refereed Article
Keywords:malate, rice, roots, photosynthesis, stress, channel, OsALMT4
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Summer Grains and Oilseeds
Objective Field:Rice
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:128649
Year Published:2018
Web of Science® Times Cited:1
Deposited By:TIA - Research Institute
Deposited On:2018-10-04
Last Modified:2019-03-21
Downloads:34 View Download Statistics

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