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Fish gill damage by harmful microalgae newly explored by microelectrode ion flux estimation techniques


Mardones, JI and Shabala, L and Shabala, S and Dorantes-Aranda, JJ and Seger, A and Hallegraeff, GM, Fish gill damage by harmful microalgae newly explored by microelectrode ion flux estimation techniques, Harmful Algae, 80 pp. 55-63. ISSN 1568-9883 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 Elsevier B.V.

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DOI: doi:10.1016/j.hal.2018.09.004


Harmful algal blooms (HAB) are responsible for massive mortalities of wild and aquacultured fish due to noticeable gill damage, but the precise fish-killing mechanisms remain poorly understood. A non-invasive microelectrode ion flux estimation (MIFE) technique was successfully applied to assess changes in membrane-transport processes in a model fish gill cell line exposed to harmful microplankton. Net Ca2+, H+, K+ ion fluxes in the rainbow trout cell line RTgill-W1 were monitored before and after addition of lysed cells of this Paralytic Shellfish Toxins (PST) producer along with purified endocellular dinoflagellate PST. It was demonstrated that PST alone do not play a role in fish gill damage during A. catenella outbreaks as previously thought, but that other ichthyotoxic metabolites from lysed algal cells (i.e. lipid peroxidation products or other unknown metabolites) result in net K+ efflux from fish gill cells and thereby gill cell death.

Item Details

Item Type:Refereed Article
Keywords:microelectrode ion flux estimation, fish-killing, Alexandrium catenella, ichthyotoxins, PST, RTgill-W1, cell membrane ion fluxes
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Phycology (incl. marine grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Mardones, JI (Mr Jorge Mardones)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Dorantes-Aranda, JJ (Dr Juan Dorantes Aranda)
UTAS Author:Seger, A (Mr Andreas Seger)
UTAS Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:128549
Year Published:2018
Funding Support:Australian Research Council (DP130102859)
Web of Science® Times Cited:5
Deposited By:Ecology and Biodiversity
Deposited On:2018-09-28
Last Modified:2018-11-15

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