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TRPV1 mediates capsaicin-stimulated metabolic activity but not cell death or inhibition of interleukin-1β release in human THP-1 monocytes
Citation
Kunde, DA and Yingchoncharoen, J and Jurkovic, S and Geraghty, DP, TRPV1 mediates capsaicin-stimulated metabolic activity but not cell death or inhibition of interleukin-1β release in human THP-1 monocytes, Toxicology and Applied Pharmacology, 360 pp. 9-17. ISSN 0041-008X (2018) [Refereed Article]
Copyright Statement
© 2018 Elsevier Inc.
DOI: doi:10.1016/j.taap.2018.09.025
Abstract
Human monocytes and dendritic cells express transient receptor potential vanilloid 1 (TRPV1) which may play a role in mediating the inflammatory, immune and cancer surveillance responses of these cells. The aim of the present study was to investigate TRPV1 expression and function in THP-1 monocytic cells. RT-PCR and Western blot were used to detect TRPV1. The metabolic activity and viability of THP-1 cells following exposure to vanilloids was assessed using resorufin production from rezazurin. Cytokine release was measured using ELISA. TRPV1 was expressed in cultured THP-1 monocytic cells and naïve monocytes. Lower concentrations (<250 μM) of capsaicin, but not other putative TRPV1 agonists, were shown to stimulate cell metabolic activity, whereas at concentrations >250 μM, all agonists decreased metabolic activity. Capsaicin-stimulated THP-1 metabolic activity was blocked by the TRPV1 antagonist, 5-iodo-resiniferatoxin (5’-IRTX), whereas the decline in resorufin production by THP-1 cells at higher capsaicin concentrations (due to cell death), was not affected by 5’-IRTX. Finally, capsaicin (≤125 μM) significantly increased lipopolysaccharide-stimulated IL-6 and TNF-α release from THP-1 cells, whereas phytohaemagglutinin-stimulated IL-1β, TNF-α, MCP-1 and IL-6 release were concentration-dependently inhibited by capsaicin. Modulation of IL-1β release was not TRPV1 mediated. Overall, these results show that functional TRPV1 channels are present in naïve monocytes and THP-1 cells, and when activated, increase cell metabolic activity. In addition, capsaicin modifies cytokine release from THP-1 cells and induces cell death, most likely by a mechanism that is independent of TRPV1 activation.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | TRPV1, capsaicin, cytokines, immunomodulation, monocytes |
| Research Division: | Medical and Health Sciences |
| Research Group: | Cardiorespiratory Medicine and Haematology |
| Research Field: | Haematology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Blood Disorders |
| UTAS Author: | Kunde, DA (Dr Dale Kunde) |
| UTAS Author: | Jurkovic, S (Mr Sasa Jurkovic) |
| UTAS Author: | Geraghty, DP (Professor Dominic Geraghty) |
| ID Code: | 128494 |
| Year Published: | 2018 |
| Deposited By: | Health Sciences |
| Deposited On: | 2018-09-25 |
| Last Modified: | 2018-11-30 |
| Downloads: | 0 |
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