Thread based electrofluidic platform for direct metabolite analysis in complex samples
Cabot, JM and Breadmore, MC and Paull, B, Thread based electrofluidic platform for direct metabolite analysis in complex samples, Analytica Chimica Acta, 1000 pp. 283-292. ISSN 0003-2670 (2018) [Refereed Article]
The application of electrophoresis upon commercial threads is investigated for development of low-cost diagnostics assays, designed for the matrix separation and quantification of low abundance metabolites in complex samples – in this work riboflavin in human urine. Zone electrophoresis was evaluated upon 8 commercially available threads, with several synthetic threads exhibiting higher electroosmotic flow (EOF) and increased electrophoretic mobility of the rhodamine 6G, rhodamine B, and fluorescein. Of those tested, a nylon bundle was selected as the best platform, offering less band dispersion and higher resolution, a high relative EOF, whilst minimising the contribution of joule heating. A novel 3D printed platform was designed, based on a modular system, facilitating the electrophoresis process and rapid assembly, whilst offering the potential for multiplexed analysis or investigation of more complex systems. Using the thread-based electrophoresis system, riboflavin was determined in less than 2 minutes. The device exhibited a linear working range from 0.1 to 15 µg/mL of riboflavin in urine, and was in good agreement with capillary electrophoresis measurements.
electrofluidics, thread-based assays, metabolite analysis, microfluidic thread based analytical device, thread electrophoresis, riboflavin, urine analysis, 3D printed platform