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Development of 15 nuclear EST microsatellite markers for the paleoendemic conifer Pherosphaera hookeriana (Podocarpaceae)

Citation

Worth, JRP and Marthick, JR and Rossetto, M and Cohen, J and Bourke, G and Jordan, GJ, Development of 15 nuclear EST microsatellite markers for the paleoendemic conifer Pherosphaera hookeriana (Podocarpaceae), Applications in Plant Sciences, 6, (6) Article e01160. ISSN 2168-0450 (2018) [Refereed Article]


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Copyright Statement

Copyright 2018 Worth et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1002/aps3.1160

Abstract

Premise of the Study: Nuclear microsatellite markers were developed for population genetic analysis of the threatened paleoendemic conifer Pherosphaera hookeriana (Podocarpaceae).

Methods and Results: Fifteen variable loci were identified showing one to 13 alleles per population, with seven loci displaying at least four alleles in all populations, and the average number of alleles per locus ranging from 4.80 to 5.93 per population. Levels of observed heterozygosity per locus varied from 0.00 to 0.91, while average heterozygosity across all loci varied from 0.54 to 0.63 between populations. All loci also amplified in the endangered congener P. fitzgeraldii, but only five of the loci had more than one allele.

Conclusions: These 15 loci are the first microsatellite markers developed in the genus Pherosphaera. These loci will be useful for investigating the species' extant genetic diversity and structure, the impact of past environmental change, and the significance of asexual reproduction.

Item Details

Item Type:Refereed Article
Keywords:Pherosphaera, Podocarpaceae, RNA‐seq, Tasmania, conifer, next‐generation sequencing, paleoendemic
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Cell and Molecular Biology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Marthick, JR (Mr James Marthick)
Author:Jordan, GJ (Associate Professor Greg Jordan)
ID Code:128159
Year Published:2018
Deposited By:Menzies Institute for Medical Research
Deposited On:2018-09-06
Last Modified:2018-10-08
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