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The Right Rodent for the Job: Infarct Variability Between Strains and Its Impact on Logistics of Experimental Animal Studies

Citation

Rewell, S and Howells, DW, The Right Rodent for the Job: Infarct Variability Between Strains and Its Impact on Logistics of Experimental Animal Studies, Neuroprotective Therapy for Stroke and Ischemic Disease, Springer International Publishing, PA Lapchak and JH Zhang (ed), Switzerland, pp. 667-687. ISBN 978-3-319-45344-6 (2017) [Research Book Chapter]

Copyright Statement

Copyright 2017 Springer International Publishing Switzerland

DOI: doi:10.1007/978-3-319-45345-3_28

Abstract

This chapter will discuss the variability in infarct size after ischaemic stroke in rat models of stroke, drawing example from our experience with the thread occlusion model. We will describe how the neuroprotective effect of a novel treatment diminished over the course of our testing, with post hoc analysis revealing wide variability in infarct volume in the experiments where the treatment was not shown to be protective. Application of various inclusion criteria failed to reduce variability, only reducing the number of animals. We then compared infarct variability in the Sprague-Dawley strain to other strains of rat used in our laboratory. The spontaneously hypertensive rat proved to be the most consistent strain of rat, having the least variable infarct volume, and stroke being successfully induced in all animals. The ability to include more animals in experimental groups is advantageous in terms of the absolute number of animals used, the time an experiment will take to complete and the cost of preclinical research.

Item Details

Item Type:Research Book Chapter
Keywords:rat, stroke, variability, exclusion criteria
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
UTAS Author:Howells, DW (Professor David Howells)
ID Code:128036
Year Published:2017
Deposited By:Medicine
Deposited On:2018-08-28
Last Modified:2018-09-06
Downloads:0

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