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Uses and misuses of progress curve analysis in enzyme kinetics

Citation

Nikolova, N and Tenekedjiev, K and Kolev, K, Uses and misuses of progress curve analysis in enzyme kinetics, Central European Journal of Biology, 3, (4) pp. 354-350. ISSN 1895-104X (2008) [Refereed Article]


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Copyright Statement

Copyright 2008 The Author(s) Licensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) https://creativecommons.org/licenses/by-nc-nd/3.0/

DOI: doi:10.2478/s11535-008-0035-4

Abstract

Progress curve analysis is a convenient tool for the characterization of enzyme action: a single reaction mixture provides multiple experimental measured points for continuously varying amounts of substrates and products with exactly the same enzyme and modulator concentrations. The determination of kinetic parameters from the progress curves, however, requires complex mathematical evaluation of the time-course data. Some freely available programs (e.g. FITSIM, DYNAFIT) are widely applied to fit kinetic parameters to user-defined enzymatic mechanisms, but users often overlook the stringent requirements of the analytic procedures for appropriate design of the input experiments. Flaws in the experimental setup result in unreliable parameters with consequent misinterpretation of the biological phenomenon under study. The present commentary suggests some helpful mathematical tools to improve the analytic procedure in order to diagnose major errors in concept and design of kinetic experiments.

Item Details

Item Type:Refereed Article
Keywords:enzyme kinetics
Research Division:Information and Computing Sciences
Research Group:Information Systems
Research Field:Decision Support and Group Support Systems
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Information and Computing Sciences
Author:Nikolova, N (Professor Nataliya Nikolova)
Author:Tenekedjiev, K (Professor Kiril Tenekedjiev)
ID Code:127967
Year Published:2008
Web of Science® Times Cited:7
Deposited By:AMC Governance Office
Deposited On:2018-08-26
Last Modified:2018-09-07
Downloads:12 View Download Statistics

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