A new, multisyringe flow injection set-up has been developed for the completely automated determination of trace thiazide compounds with diuretic action in different types of samples. The proposed instrumental set-up exploits for the first time, a low pressure on-line solid phase extraction–liquid chromatography–chemiluminescence detection method. This novel combination of sample treatments in flow systems expands the current applicability of low pressure liquid chromatography due to the isolation/preconcentration of the target compounds, besides high selectivity and sensitivity.

For the determination of three thiazide compounds named hydroflumethiazide, furosemide and bendroflumethiazide, the proposed set-up provided with the preconcentration of only 1 mL of sample, limits of detection of 3, 60 and 40 μg L⁻¹, respectively. Furthermore wide linear dynamic ranges of 6–4000, 140–20,000 and 90–40,000 μg L⁻¹, respectively, were obtained. Besides of this, a high injection throughput of 12 h⁻¹ was also achieved. As in sports, thiazide diuretics are prohibited substances, the proposed method has been applied to their determination in urine samples. Furthermore the potential of the proposed method as a fast-screening approach for emerging contaminants in waters has been also tested by applying it to well water and leachates from a solid waste landfill.

Item Type:	Refereed Article
Keywords:	flow analysis, solid phase extraction, monolithic column, multisyringe chromatography, chemiluminescence, thiazide diuretics
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Flow analysis
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Maya, F (Mr Fernando Maya Alejandro)
ID Code:	127867
Year Published:	2010
Web of Science® Times Cited:	49
Deposited By:	Chemistry
Deposited On:	2018-08-19
Last Modified:	2018-09-11
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