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Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles


Lv, Y and Maya Alejandro, F and Frechet, JMJ and Svec, F, Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles, Journal of Chromatography A, 1261 pp. 121-128. ISSN 0021-9673 (2012) [Refereed Article]

Copyright Statement

2012 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.chroma.2012.04.007


A new approach to the preparation of porous polymer monoliths with enhanced coverage of pore surface with gold nanoparticles has been developed. First, a generic poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was reacted with cystamine followed by the cleavage of its disulfide bonds with tris(2-carboxylethyl)phosphine, which liberated the desired thiol groups. Dispersions of gold nanoparticles with sizes varying from 5 to 40 nm were then pumped through the functionalized monoliths. The materials were then analyzed using both energy dispersive X-ray spectroscopy and thermogravimetric analysis. We found that the quantity of attached gold was dependent on the size of nanoparticles, with the maximum attachment of more than 60 wt% being achieved with 40 nm nanoparticles. Scanning electron micrographs of the cross sections of all the monoliths revealed the formation of a non-aggregated, homogenous monolayer of nanoparticles. The surface of the bound gold was functionalized with 1-octanethiol and 1-octadecanethiol, and these monolithic columns were used successfully for the separations of proteins in reversed phase mode. The best separations were obtained using monoliths modified with 15, 20, and 30 nm nanoparticles since these sizes produced the most dense coverage of pore surface with gold.

Item Details

Item Type:Refereed Article
Keywords:polymer monoliths, gold nanoparticles, protein separation, reversed phase
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Maya Alejandro, F (Mr Fernando Maya Alejandro)
ID Code:127858
Year Published:2012
Web of Science® Times Cited:105
Deposited By:Chemistry
Deposited On:2018-08-19
Last Modified:2018-09-07

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