Lamprou, A and Wang, H and Saeed, A and Svec, F and Britt, D and Maya, F, Preparation of highly porous coordination polymer coatings on macroporous polymer monoliths for enhanced enrichment of phosphopeptides, Journal of Visualized Experiments, 101 Article e52926. ISSN 1940-087X (2015) [Refereed Article]
Copyright 2015 Journal of Visualized Experiments
Official URL: https://www.jove.com/video/52926/preparation-highl...
The developed preparation scheme is easily adapted to a capillary column format. The functional porous polymer is prepared as a self-contained single-block porous monolith within the capillary, yielding a flow-through separation device with excellent flow permeability and modest backpressure. The metal-organic polymer hybrid column showed excellent performance for the enrichment of phosphopeptides from digested proteins and their subsequent detection using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presented experimental protocol is highly versatile, and can be easily implemented to different organic polymer supports and coatings with a plethora of porous coordination polymers and metal-organic frameworks for multiple purification and/or separation applications.
|Item Type:||Refereed Article|
|Keywords:||metal-organic frameworks, polymer monoliths|
|Research Division:||Chemical Sciences|
|Research Group:||Analytical Chemistry|
|Research Field:||Separation Science|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding Knowledge|
|Objective Field:||Expanding Knowledge in the Chemical Sciences|
|UTAS Author:||Maya, F (Mr Fernando Maya Alejandro)|
|Web of Science® Times Cited:||6|
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