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Automated solid-phase extraction of phenolic acids using layered double hydroxide–alumina–polymer disks


Ghani, M and Cabello, CP and Saraji, M and Estela, JM and Cerda, V and Palomino, GT and Maya, F, Automated solid-phase extraction of phenolic acids using layered double hydroxide-alumina-polymer disks, Journal of Separation Science, 41, (9) pp. 2012-2019. ISSN 1615-9306 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/jssc.201701420


The application of layered double hydroxide-Al2 O3 -polymer mixed-matrix disks for solid-phase extraction is reported for the first time. Al2 O3 is embedded in a polymer matrix followed by an in situ metal-exchange process to obtain a layered double hydroxide-Al2 O3 -polymer mixed-matrix disk with excellent flow-through properties. The extraction performance of the prepared disks is evaluated as a proof of concept for the automated extraction using sequential injection analysis of organic acids (p-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid, gallic acid) following an anion-exchange mechanism. After the solid-phase extraction, phenolic acids were quantified by reversed-phase high-performance liquid chromatography with diode-array detection using a core-shell silica-C18 stationary phase and isocratic elution (acetonitrile/0.5% acetic acid in pure water, 5:95, v/v). High sensitivity and reproducibility were obtained with limits of detection in the range of 0.12-0.25 μg/L (sample volume, 4 mL), and relative standard deviations between 2.9 and 3.4% (10 μg/L, n = 6). Enrichment factors of 34-39 were obtained. Layered double hydroxide-Al2 O3 -polymer mixed-matrix disks had an average lifetime of 50 extractions. Analyte recoveries ranged from 93 to 96% for grape juice and nonalcoholic beer samples.

Item Details

Item Type:Refereed Article
Keywords:membranes, chromatography, automated sample treatment, high-performance liquid chromatography, layered double hydroxides, phenolic acids, solid-phase extraction
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Maya, F (Mr Fernando Maya Alejandro)
ID Code:127825
Year Published:2018
Web of Science® Times Cited:13
Deposited By:Chemistry
Deposited On:2018-08-19
Last Modified:2018-09-20

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