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Pharmacological inhibition of store-operated calcium entry in MDA-MB-468 basal A breast cancer cells: consequences on calcium signalling, cell migration and proliferation

Citation

Azimi, I and Bong, AH and Poo, GXH and Armitage, K and Lok, D and Roberts-Thomson, SJ and Monteith, GR, Pharmacological inhibition of store-operated calcium entry in MDA-MB-468 basal A breast cancer cells: consequences on calcium signalling, cell migration and proliferation, Cellular and Molecular Life Sciences, 75, (24) pp. 4525-4537. ISSN 1420-682X (2018) [Refereed Article]

Copyright Statement

Springer Nature Switzerland AG 2018

DOI: doi:10.1007/s00018-018-2904-y

Abstract

Store-operated Ca2+ entry is a pathway that is remodelled in a variety of cancers, and altered expression of the components of store-operated Ca2+ entry is a feature of breast cancer cells of the basal molecular subtype. Studies of store-operated Ca2+ entry in breast cancer cells have used non-specifc pharmacological inhibitors, complete depletion of intracellular Ca2+ stores and have mostly focused on MDA-MB-231 cells (a basal B breast cancer cell line). These studies compared the efects of the selective store-operated Ca2+ entry inhibitors Synta66 and YM58483 (also known as BTP2) on global cytosolic free Ca2+ ([Ca2+]CYT) changes induced by physiological stimuli in a diferent breast cancer basal cell line model, MDA-MB-468. The efects of these agents on proliferation as well as serum and epidermal growth factor (EGF) induced migration were also assessed. Activation with the purinergic receptor activator adenosine triphosphate, produced a sustained increase in [Ca2+]CYT that was entirely dependent on store-operated Ca2+ entry. The protease activated receptor 2 activator, trypsin, and EGF also produced Ca2+ infux that was sensitive to both Synta66 and YM58483. Serum-activated migration of MDA-MB-468 breast cancer cells was sensitive to both store-operated Ca2+ inhibitors. However, proliferation and EGF-activated migration was diferentially afected by Synta66 and YM58483. These studies highlight the need to defne the exact mechanisms of action of diferent store-operated calcium entry inhibitors and the impact of such diferences in the control of tumour progression pathways.

Item Details

Item Type:Refereed Article
Keywords:pharmacology, cancer, Orai1, store-operated calcium entry, Synta66, YM58483
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Basic pharmacology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Azimi, I (Dr Iman Azimi)
ID Code:127768
Year Published:2018
Web of Science® Times Cited:29
Deposited By:Pharmacy
Deposited On:2018-08-15
Last Modified:2019-03-05
Downloads:0

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