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Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters

Citation

Anderson, K and Taylor, DA and Thompson, EL and Melwani, AR and Nair, SV and Raftos, DA, Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters, PloS ONE, 10, (3) Article e0118839. ISSN 1932-6203 (2015) [Refereed Article]


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Copyright Statement

Copyright 2015 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0118839

Abstract

Many microarray and suppression subtractive hybridization (SSH) studies have analyzed the effects of environmental stress on gene transcription in marine species. However, there have been no unifying analyses of these data to identify common stress response pathways. To address this shortfall, we conducted a meta-analysis of 14 studies that investigated the effects of different environmental stressors on gene expression in oysters. The stressors tested included chemical contamination, hypoxia and infection, as well as extremes of temperature, pH and turbidity. We found that the expression of over 400 genes in a range of oyster species changed significantly after exposure to environmental stress. A repeating pattern was evident in these transcriptional responses, regardless of the type of stress applied. Many of the genes that responded to environmental stress encoded proteins involved in translation and protein processing (including molecular chaperones), the mitochondrial electron transport chain, anti-oxidant activity and the cytoskeleton. In light of these findings, we put forward a consensus model of sub-cellular stress responses in oysters.

Item Details

Item Type:Refereed Article
Keywords:meta-analysis, environmental stress, metabolism, stress response, invertebrate biology
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Systems Biology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Oysters
Author:Anderson, K (Dr Kelli Anderson)
ID Code:127616
Year Published:2015
Web of Science® Times Cited:27
Deposited By:Fisheries and Aquaculture
Deposited On:2018-08-06
Last Modified:2018-09-05
Downloads:7 View Download Statistics

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