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Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters
Citation
Anderson, K and Taylor, DA and Thompson, EL and Melwani, AR and Nair, SV and Raftos, DA, Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters, PloS ONE, 10, (3) Article e0118839. ISSN 1932-6203 (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1371/journal.pone.0118839
Abstract
Many microarray and suppression subtractive hybridization (SSH) studies have analyzed
the effects of environmental stress on gene transcription in marine species. However, there
have been no unifying analyses of these data to identify common stress response pathways.
To address this shortfall, we conducted a meta-analysis of 14 studies that investigated
the effects of different environmental stressors on gene expression in oysters. The
stressors tested included chemical contamination, hypoxia and infection, as well as extremes
of temperature, pH and turbidity. We found that the expression of over 400 genes in
a range of oyster species changed significantly after exposure to environmental stress. A repeating
pattern was evident in these transcriptional responses, regardless of the type of
stress applied. Many of the genes that responded to environmental stress encoded proteins
involved in translation and protein processing (including molecular chaperones), the mitochondrial
electron transport chain, anti-oxidant activity and the cytoskeleton. In light of
these findings, we put forward a consensus model of sub-cellular stress responses
in oysters.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | meta-analysis, environmental stress, metabolism, stress response, invertebrate biology |
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and Cell Biology |
| Research Field: | Systems Biology |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Fisheries - Aquaculture |
| Objective Field: | Aquaculture Oysters |
| UTAS Author: | Anderson, K (Dr Kelli Anderson) |
| ID Code: | 127616 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 32 |
| Deposited By: | Fisheries and Aquaculture |
| Deposited On: | 2018-08-06 |
| Last Modified: | 2018-09-05 |
| Downloads: | 35 View Download Statistics |
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