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Methods for In Vivo CRISPR/Cas Editing of the Adult Murine Retina
Citation
Hung, SS and Li, F and Wang, J-H and King, AE and Bui, BV and Liu, G, Methods for In Vivo CRISPR/Cas Editing of the Adult Murine Retina, Retinal Gene Therapy: Methods and Protocols, Humana Press, CJF Boon, J Wijnholds (ed), United States, pp. 113-133. ISBN 978-1-4939-7521-1 (2018) [Other Book Chapter]
Copyright Statement
Copyright 2018 Springer Science+Business Media LLC
DOI: doi:10.1007/978-1-4939-7522-8_9
Abstract
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) is used by some bacteria and most archaea to protect against viral phage intrusion and has recently been adapted to allow for efficient editing of the mammalian genome. Whilst CRISPR/Cas-based technology has been used to modify genes in mammalian cells in vitro, delivery of CRISPR/Cas system into mammalian tissue and/or organs is more difficult and often requires additional vectors. With the use of adeno-associated virus (AAV) gene delivery system, active CRISPR/Cas enzyme can be maintained for an extended period of time and enable efficient editing of genome in the retina in vivo. Herein we outline the method to edit the genome in mouse retina using a dual AAV vector-mediated CRISPR/Cas9 system.
Item Details
Item Type: | Other Book Chapter |
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Keywords: | CRISPR/Cas9, genome editing, gene delivery, AAV, retina |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical biotechnology |
Research Field: | Gene and molecular therapy |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Li, F (Dr Fan Li) |
UTAS Author: | King, AE (Professor Anna King) |
UTAS Author: | Liu, G (Associate Professor Guei-Sheung Liu) |
ID Code: | 127341 |
Year Published: | 2018 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2018-07-23 |
Last Modified: | 2019-02-21 |
Downloads: | 0 |
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