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An anion conductance, the essential component of the hydroxyl-radical-induced ion current in plant roots

Citation

Pottosin, I and Zepeda-Jazo, I and Bose, J and Shabala, S, An anion conductance, the essential component of the hydroxyl-radical-induced ion current in plant roots, International Journal of Molecular Sciences, 19 Article 897. ISSN 1422-0067 (2018) [Refereed Article]


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Copyright Statement

Copyright 2018 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms19030897

Abstract

Oxidative stress signaling is essential for plant adaptation to hostile environments. Previous studies revealed the essentiality of hydroxyl radicals (HO•)-induced activation of massive K+ efflux and a smaller Ca2+ influx as an important component of plant adaptation to a broad range of abiotic stresses. Such activation would modify membrane potential making it more negative. Contrary to these expectations, here, we provide experimental evidence that HO• induces a strong depolarization, from −130 to −70 mV, which could only be explained by a substantial HO•-induced efflux of intracellular anions. Application of Gd3+ and NPPB, non-specific blockers of cation and anion conductance, respectively, reduced HO•-induced ion fluxes instantaneously, implying a direct block of the dual conductance. The selectivity of an early instantaneous HO•-induced whole cell current fluctuated from more anionic to more cationic and vice versa, developing a higher cation selectivity at later times. The parallel electroneutral efflux of K+ and anions should underlie a substantial leak of the cellular electrolyte, which may affect the cell’s turgor and metabolic status. The physiological implications of these findings are discussed in the context of cell fate determination, and ROS and cytosolic K+ signaling.

Item Details

Item Type:Refereed Article
Keywords:ion channel, membrane transport, anion conductance, electrolyte leakage, hydroxyl radical, membrane potential, MIFE, oxidative stress, patch-clamp
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
UTAS Author:Pottosin, I (Professor Igor Pottosin)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:127308
Year Published:2018
Deposited By:Agriculture and Food Systems
Deposited On:2018-07-20
Last Modified:2019-03-05
Downloads:22 View Download Statistics

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