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Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness

Citation

Minina, EA and Moschou, PN and Vetukuri, RR and Sanchez-Vera, V and Cardoso, C and Liu, Q and Elasnder, PH and Dalman, K and Beganovic, M and Yilmaz, JL and Marmon, S and Shabala, L and Suarez, MF and Ljung, K and Novak, O and Shabala, S and Stymne, S and Hofius, D and Bozhkov, PV, Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness, Journal of Experimental Botany, 69, (6) pp. 1415-1432. ISSN 0022-0957 (2018) [Refereed Article]


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Copyright 2017 the Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1093/jxb/ery010

Abstract

Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic compartment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5, and Atg8 with lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these systems causes autophagy-deficient phenotypes with reduced fitness and longevity. We show that Atg5 and the E1-like enzyme, Atg7, are rate-limiting components of Atg8–PE conjugation in Arabidopsis. Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation, and autophagic flux. It also induces transcriptional changes opposite to those observed in atg5 and atg7 mutants, favoring stress resistance and growth. As a result, ATG5- or ATG7-overexpressing plants exhibit increased resistance to necrotrophic pathogens and oxidative stress, delayed aging and enhanced growth, seed set, and seed oil content. This work provides an experimental paradigm and mechanistic insight into genetic stimulation of autophagy in planta and shows its efficiency for improving plant productivity.

Item Details

Item Type:Refereed Article
Keywords:autphagy, stress, signalling' adaptation
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:127307
Year Published:2018
Web of Science® Times Cited:19
Deposited By:Agriculture and Food Systems
Deposited On:2018-07-20
Last Modified:2019-03-05
Downloads:21 View Download Statistics

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