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A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex

Citation

Shin, Y and Perera, AP and Tang, WY and Fu, DL and Liu, Q and Sheng, JK and Gu, Z and Lee, TY and Barkham, T and Kyoung Park, M, A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex, Biosensors & bioelectronics, 68 pp. 390-396. ISSN 0956-5663 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.bios.2015.01.030

Abstract

Global tuberculosis (TB) control is hampered by cost and slow or insensitive diagnostic methods to be used for TB diagnosis in clinic. Thus, TB still remains a major global health problem. The failure to rapidly and accurately diagnose of TB has posed significant challenges with consequent secondary resistance and ongoing transmission. We developed a rapid Mycobacterium tuberculosis (MTB) amplification/detection method, called MTB isothermal solid-phase amplification/detection (MTB-ISAD), that couples isothermal solid-phase amplification and a silicon biophotonics-based detection sensor to allow the simultaneous amplification and detection of MTB in a label-free and real-time manner. We validated the clinical utility of the MTB-ISAD assay by detecting MTB nucleic acid in sputum samples from 42 patients. We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (<20 min), highly sensitive, accurate (>90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling. The MTB-ISAD assay has improved time-efficiency, affordability, and sensitivity compared with many existing methods. Therefore, it is potentially adaptable for better diagnosis across various clinical applications.

Item Details

Item Type:Refereed Article
Keywords:Infectious disease, Silicon biophotonicsensor, Mycobacterium tuberculosis ,DNAamplification/detection
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Infectious Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
UTAS Author:Perera, AP (Mrs Agampodi Perera)
ID Code:127175
Year Published:2015
Web of Science® Times Cited:20
Deposited By:Health Sciences
Deposited On:2018-07-16
Last Modified:2018-08-23
Downloads:0

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