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Expression of animal CED-9 anti-apoptotic gene in tobacco modiWes plasma membrane ion Xuxes in response to salinity and oxidative stress

Citation

Shabala, S and Cuin, TA and Prismall, L and Nemchinov, LG, Expression of animal CED-9 anti-apoptotic gene in tobacco modiWes plasma membrane ion Xuxes in response to salinity and oxidative stress, Planta, 227 pp. 189-197. ISSN 0032-0935 (2007) [Refereed Article]


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DOI: doi:10.1007/s00425-007-0606-z

Abstract

Apoptosis, one form of programmed cell death (PCD), plays an important role in mediating plant adaptive responses to the environment. Recent studies suggest that expression of animal anti-apoptotic genes in transgenic plants may signiWcantly improve a plantís ability to tolerate a variety of biotic and abiotic stresses. The underlying cellular mechanisms of this process remain unexplored. In this study, we investigated speciWc ion Xux "signatures" in Nicotiana benthamiana plants transiently expressing CED-9 anti-apoptotic gene and undergoing salt- and oxidative stresses. Using a range of electrophysiological techniques, we show that expression of CED-9 increased plant salt and oxidative stress tolerance by altering K+ and H+ Xux patterns across the plasma membrane. Our data shows that PVX/ CED-9 plants are capable of preventing stress-induced K+ eZux from mesophyll cells, so maintaining intracellular K+ homeostasis. We attribute these eVects to the ability of CED-9 to control at least two types of K+-permeable channels; outward-rectifying depolarization-activating K+ channels (KOR) and non-selective cation channels (NSCC). A possible scenario linking CED-9 expression and ionic relations in plant cell is suggested. To the best of our knowledge, this study is the Wrst to link "ion Xux signatures" and mechanisms involved in regulation of PCD in plants.

Item Details

Item Type:Refereed Article
Keywords:membrane transport, Apoptosis, programmed cell death, salinity, oxidative stress
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Management of Gaseous Waste from Plant Production (excl. Greenhouse Gases)
Author:Shabala, S (Professor Sergey Shabala)
Author:Cuin, TA (Dr Tracey Cuin)
Author:Prismall, L (Mrs Luke Prismall)
ID Code:127013
Year Published:2007
Web of Science® Times Cited:66
Deposited By:Agriculture and Food Systems
Deposited On:2018-07-06
Last Modified:2018-07-09
Downloads:0

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