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Identification of the transporter responsible for sucrose accumulation in sugar beet taproots

Citation

Jung, B and Ludewig, F and Schulz, A and Meibner, G and Wostefeld, N and Flugge, U-I and Pommerrenig, B and Wirsching, P and Sauer, N and Koch, W and Sommer, F and Muhlhaus, T and Schroda, M and Cuin, TA and Graus, D and Marten, I and Hedrich, R and Neuhaus, HE, Identification of the transporter responsible for sucrose accumulation in sugar beet taproots, Nature Plants, 1 Article 14001. ISSN 2055-026X (2015) [Refereed Article]


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DOI: doi:10.1038/nplants.2014.1

Abstract

Sugar beet provides around one third of the sugar consumed worldwide and serves as a significant source of bioenergy in the form of ethanol. Sucrose accounts for up to 18% of plant fresh weight in sugar beet. Most of the sucrose is concentrated in the taproot, where it accumulates in the vacuoles. Despite 30 years of intensive research, the transporter that facilitates taproot sucrose accumulation has escaped identification. Here, we combine proteomic analyses of the taproot vacuolar membrane, the tonoplast, with electrophysiological analyses to show that the transporter BvTST2.1 is responsible for vacuolar sucrose uptake in sugar beet taproots. We show that BvTST2.1 is a sucrose-specific transporter, and present evidence to suggest that it operates as a proton antiporter, coupling the import of sucrose into the vacuole to the export of protons. BvTST2.1 exhibits a high amino acid sequence similarity to members of the tonoplast monosaccharide transporter family in Arabidopsis, prompting us to rename this group of proteins ‘tonoplast sugar transporters’. The identification of BvTST2.1 could help to increase sugar yields from sugar beet and other sugar-storing plants in future breeding programs.

Item Details

Item Type:Refereed Article
Keywords:sugar beet, vacuole, sucrose, Tonoplast Vacuolar Transporter, Patch clamp
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Management of Gaseous Waste from Plant Production (excl. Greenhouse Gases)
Author:Cuin, TA (Dr Tracey Cuin)
ID Code:127010
Year Published:2015
Web of Science® Times Cited:32
Deposited By:Agriculture and Food Systems
Deposited On:2018-07-06
Last Modified:2018-07-09
Downloads:0

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