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SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner

Citation

Jaborsky, M and Maierhofer, T and Olbrich, A and Escalante-Perez, M and Muller, HM and Simon, J and Krol, E and Cuin, TA and Fromm, J and Ache, P and Geiger, D and Hedrich, R, SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner, New Phytologist, 210, (3) pp. 922-933. ISSN 1469-8137 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 The Authors New Phytologist Copyright 2018 New Phytologist Trust

DOI: doi:10.1111/nph.13841

Abstract

  • Extrafloral nectaries secrete a sweet sugar cocktail that lures predator insects for protection from foraging herbivores. Apart from sugars and amino acids, the nectar contains the anions chloride and nitrate. Recent studies with Populus have identified a type of nectary covered by apical bipolar epidermal cells, reminiscent of the secretory brush border epithelium in animals. Border epithelia operate transepithelial anion transport, which is required for membrane potential and/or osmotic adjustment of the secretory cells.
  • In search of anion transporters expressed in extrafloral nectaries, we identified PttSLAH3 (Populus tremula x Populus tremuloides SLAC1 Homologue3), an anion channel of the SLAC/SLAH family.
  • When expressed in Xenopus oocytes, PttSLAH3 displayed the features of a voltagedependent anion channel, permeable to both nitrate and chloride. In contrast to the Arabidopsis SLAC/SLAH family members, the poplar isoform PttSLAH3 is independent of phosphorylation activation by protein kinases. To understand the basis for the autonomous activity of the poplar SLAH3, we generated and expressed chimera between kinaseindependent PttSLAH3 and kinase-dependent Arabidopsis AtSLAH3. We identified the N-terminal tail and, to a lesser extent, the C-terminal tail as responsible for PttSLAH3 kinase- (in)dependent action.
  • This feature of PttSLAH3 may provide the secretory cell with a channel probably controlling long-term nectar secretion.
  • Item Details

    Item Type:Refereed Article
    Keywords:anion channels, extrafloralnectaries, poplar, SLAC/SLAH, S-type anionchannels, transepithelial transport
    Research Division:Biological Sciences
    Research Group:Plant Biology
    Research Field:Plant Physiology
    Objective Division:Plant Production and Plant Primary Products
    Objective Group:Environmentally Sustainable Plant Production
    Objective Field:Management of Gaseous Waste from Plant Production (excl. Greenhouse Gases)
    Author:Cuin, TA (Dr Tracey Cuin)
    ID Code:127009
    Year Published:2016
    Web of Science® Times Cited:4
    Deposited By:Agriculture and Food Systems
    Deposited On:2018-07-06
    Last Modified:2018-08-21
    Downloads:0

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