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High V-PPase activity is beneficial under high salt loads, but detrimental without salinity

Citation

Graus, D and Konrad, KR and Bemm, F and Patir Nebioglu, MG and Lorey, C and Duscha, K and Guthoff, T and Herrmann, J and Ferjani, A and Cuin, TA and Roelfsema, MRG and Schumacher, K and Neuhaus, HE and Marten, I and Hedrich, R, High V-PPase activity is beneficial under high salt loads, but detrimental without salinity, New Phytologist, 219, (4) pp. 1421-1432. ISSN 1469-8137 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 The Authors New Phytologist Copyright 2018 New Phytologist Trust

DOI: doi:10.1111/nph.15280

Abstract

  • The membrane-bound proton-pumping pyrophosphatase (V-PPase), together with the V-type H+ -ATPase, generates the proton motive force that drives vacuolar membrane solute transport. Transgenic plants constitutively overexpressing V-PPases were shown to have improved salinity tolerance, but the relative impact of increasing PPi hydrolysis and protonpumping functions has yet to be dissected.
  • For a better understanding of the molecular processes underlying V-PPase-dependent salt tolerance, we transiently overexpressed the pyrophosphate-driven proton pump (NbVHP) in Nicotiana benthamiana leaves and studied its functional properties in relation to salt treatment by primarily using patch-clamp, impalement electrodes and pH imaging.
  • NbVHP overexpression led to higher vacuolar proton currents and vacuolar acidification. After 3 d in salt-untreated conditions, V-PPase-overexpressing leaves showed a drop in photosynthetic capacity, plasma membrane depolarization and eventual leaf necrosis. Salt, however, rescued NbVHP-hyperactive cells from cell death. Furthermore, a salt-induced rise in V-PPase but not of V-ATPase pump currents was detected in nontransformed plants.
  • The results indicate that under normal growth conditions, plants need to regulate the V-PPase pump activity to avoid hyperactivity and its negative feedback on cell viability. Nonetheless, V-PPase proton pump function becomes increasingly important under salt stress for generating the pH gradient necessary for vacuolar proton-coupled Na+ sequestration.

    • Item Details

    Item Type:Refereed Article
    Keywords:cell death, plasma membrane voltage, proton pump currents, salt, vacuolar pH, vacuolar proton-ATPase (V-ATPase), vacuolar proton-pyrophosphatase (V-PPase)
    Research Division:Biological Sciences
    Research Group:Plant Biology
    Research Field:Plant Physiology
    Objective Division:Plant Production and Plant Primary Products
    Objective Group:Environmentally Sustainable Plant Production
    Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
    UTAS Author:Cuin, TA (Dr Tracey Cuin)
    ID Code:127001
    Year Published:2018
    Web of Science® Times Cited:6
    Deposited By:Agriculture and Food Systems
    Deposited On:2018-07-06
    Last Modified:2019-03-05
    Downloads:0

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