eCite Digital Repository

Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men

Citation

Hostrup, M and Reitelseder, S and Jessen, S and Kalsen, A and Nyberg, M and Egelund, J and Kreiberg, M and Kristensen, CM and Thomassen, M and Pilegaard, H and Backer, V and Jacobson, GA and Holm, L and Bangsbo, J, Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men, Journal of Physiology, 596, (17) pp. 4121-4139. ISSN 0022-3751 (2018) [Refereed Article]


Preview
PDF
2Mb
  

Copyright Statement

Copyright 2018 The Authors. The Journal of Physiology Copyright 2018 The Physiological Society

DOI: doi:10.1113/JP275560

Abstract

The effect of beta2-adrenoceptor stimulation on skeletal muscle protein turnover and intracellular signalling is insufficiently explored in humans, particularly in association with exercise. In a randomized placebo-controlled crossover study with 12 trained men, the effect of beta2-agonist (64mg oral salbutamol) on protein turnover rates, intracellular signalling, and mRNA response in skeletal muscle was investigated 0.5-5h after quadriceps resistance exercise. Each trial was preceded by a four-day lead-in treatment period. Leg protein turnover rates were assessed by infusion of [13C6]-phenylalanine and sampling of arterial and venous blood as well as vastus lateralis muscle biopsies 0.5 and 5h after exercise. Furthermore, myofibrillar fractional synthesis rate (FSR), intracellular signalling and mRNA response were measured in muscle biopsies. Mean (95%CI) myofibrillar FSR was higher for salbutamol than placebo [0.079(0.007)vs.0.066(0.004)נh-1](p<0.05). Mean net leg phenylalanine balance 0.5-5h after exercise was [3.6(2.6)nmolmin-1נ100gLeg Lean Mass-1] higher for salbutamol than placebo (p<0.01). Phosphorylation of Akt2, CREB and PKA-substrate 0.5 and 5h after exercise as well as phosphorylation of eEF2 5 h after exercise was higher (p<0.05) for salbutamol than placebo. Calpain-1, FoxO1, myostatin and Smad3 mRNA content was higher (p<0.01) for salbutamol than placebo 0.5h after exercise, and FoxO1 and myostatin mRNA content 5h after, whereas ActivinRIIB mRNA content was lower (p<0.01) for salbutamol 5h after. These observations suggest that beta2-agonist increases protein turnover rates in skeletal muscle after resistance exercise in humans, with concomitant cAMP/PKA and Akt2 signalling, and modulation of mRNA response of growth-regulating proteins.

Item Details

Item Type:Refereed Article
Keywords:beta2-agonist salbutamol muscle doping
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Pharmaceutical Sciences
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
UTAS Author:Jacobson, GA (Associate Professor Glenn Jacobson)
ID Code:126975
Year Published:2018
Web of Science® Times Cited:6
Deposited By:Pharmacy
Deposited On:2018-07-05
Last Modified:2019-02-22
Downloads:5 View Download Statistics

Repository Staff Only: item control page