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Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men
Citation
Hostrup, M and Reitelseder, S and Jessen, S and Kalsen, A and Nyberg, M and Egelund, J and Kreiberg, M and Kristensen, CM and Thomassen, M and Pilegaard, H and Backer, V and Jacobson, GA and Holm, L and Bangsbo, J, Beta2-adrenoceptor agonist salbutamol increases protein turnover rates and alters signalling in skeletal muscle after resistance exercise in young men, Journal of Physiology, 596, (17) pp. 4121-4139. ISSN 0022-3751 (2018) [Refereed Article]
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Copyright Statement
Copyright 2018 The Authors. The Journal of Physiology Copyright 2018 The Physiological Society
DOI: doi:10.1113/JP275560
Abstract
The effect of beta2-adrenoceptor stimulation on skeletal muscle protein turnover and intracellular signalling is insufficiently explored in humans, particularly in association with exercise. In a randomized placebo-controlled crossover study with 12 trained men, the effect of beta2-agonist (6×4 mg oral salbutamol) on protein turnover rates, intracellular signalling, and mRNA response in skeletal muscle was investigated 0.5-5 h after quadriceps resistance exercise. Each trial was preceded by a four-day lead-in treatment period. Leg protein turnover rates were assessed by infusion of [13C6]-phenylalanine and sampling of arterial and venous blood as well as vastus lateralis muscle biopsies 0.5 and 5 h after exercise. Furthermore, myofibrillar fractional synthesis rate (FSR), intracellular signalling and mRNA response were measured in muscle biopsies. Mean (±95%CI) myofibrillar FSR was higher for salbutamol than placebo [0.079(±0.007) vs. 0.066(±0.004) × h-1](p<0.05). Mean net leg phenylalanine balance 0.5-5 h after exercise was [3.6(±2.6) nmol×min-1 × 100 gLeg Lean Mass-1] higher for salbutamol than placebo (p<0.01). Phosphorylation of Akt2, CREB and PKA-substrate 0.5 and 5 h after exercise as well as phosphorylation of eEF2 5 h after exercise was higher (p<0.05) for salbutamol than placebo. Calpain-1, FoxO1, myostatin and Smad3 mRNA content was higher (p<0.01) for salbutamol than placebo 0.5 h after exercise, and FoxO1 and myostatin mRNA content 5 h after, whereas ActivinRIIB mRNA content was lower (p<0.01) for salbutamol 5 h after. These observations suggest that beta2-agonist increases protein turnover rates in skeletal muscle after resistance exercise in humans, with concomitant cAMP/PKA and Akt2 signalling, and modulation of mRNA response of growth-regulating proteins.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | beta2-agonist salbutamol muscle doping |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Pharmacology and pharmaceutical sciences |
| Research Field: | Pharmaceutical sciences |
| Objective Division: | Health |
| Objective Group: | Other health |
| Objective Field: | Other health not elsewhere classified |
| UTAS Author: | Jacobson, GA (Professor Glenn Jacobson) |
| ID Code: | 126975 |
| Year Published: | 2018 |
| Web of Science® Times Cited: | 23 |
| Deposited By: | Pharmacy |
| Deposited On: | 2018-07-05 |
| Last Modified: | 2019-02-22 |
| Downloads: | 27 View Download Statistics |
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