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A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics

Citation

Vemuri, R and Shinde, T and Shastri, MD and Perera, AP and Tristram, S and Martoni, CJ and Gundamaraju, R and Ahuja, KDK and Ball, M and Eri, R, A human origin strain Lactobacillus acidophilus DDS-1 exhibits superior in vitro probiotic efficacy in comparison to plant or dairy origin probiotics, International Journal of Medical Sciences, 15, (9) pp. 840-848. ISSN 1449-1907 (2018) [Refereed Article]


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Ivyspring International Publisher. Licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/

DOI: doi:10.7150/ijms.25004

Abstract

Background: The health benefits of probiotics are well established and known to be strain-specific. However, the role of probiotics obtained from different origins and their efficacy largely remains unexplored. The aim of this study is to investigate the in vitro efficacy of probiotics from different origins.

Methods: Probiotic strains utilized in this study include Lactobacillus acidophilus DDS-1 (human origin), Bifidobacterium animalis ssp. lactis UABla-12 (human origin), L. plantarum UALp-05 (plant origin) and Streptococcus thermophilus UASt-09 (dairy origin). Screening assays such as in vitro digestion simulation, adhesion, cell viability and cytokine release were used to evaluate the probiotic potential.

Results: All strains showed good resistance in the digestion simulation process, especially DDS-1 and UALp-05, which survived up to a range of 107 to 108 CFU/mL from an initial concentration of 109 CFU/mL. Two human colonic mucus-secreting cells, HT-29 and LS174T, were used to assess the adhesion capacity, cytotoxicity/viability, and cytokine quantification. All strains exhibited good adhesion capacity. No significant cellular cytotoxicity or loss in cell viability was observed. DDS-1 and UALp-05 significantly upregulated anti-inflammatory IL-10 and downregulated pro-inflammatory TNF-α cytokine production. All the strains were able to downregulate IL-8 cytokine levels.

Conclusion: Of the 4 strains tested, DDS-1 demonstrated superior survival rates, good adhesion capacity and strong immunomodulatory effect under different experimental conditions.

Item Details

Item Type:Refereed Article
Keywords:probiotics, gut health, adhesion, gastrointestinal survival, immunomodulation
Research Division:Medical and Health Sciences
Research Group:Nutrition and Dietetics
Research Field:Nutritional Physiology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Digestive System Disorders
UTAS Author:Vemuri, R (Mr Ravichandra Vemuri)
UTAS Author:Shinde, T (Ms Tanvi Shinde)
UTAS Author:Shastri, MD (Mr Madhur Shastri)
UTAS Author:Perera, AP (Mrs Agampodi Perera)
UTAS Author:Tristram, S (Dr Stephen Tristram)
UTAS Author:Gundamaraju, R (Mr Rohit Gundamaraju)
UTAS Author:Ahuja, KDK (Dr Kiran Ahuja)
UTAS Author:Eri, R (Associate Professor Raj Eri)
ID Code:126843
Year Published:2018
Web of Science® Times Cited:5
Deposited By:Health Sciences
Deposited On:2018-06-27
Last Modified:2019-03-18
Downloads:20 View Download Statistics

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