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Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids

Citation

Barnes, TC and Izzo, C and Bertozzi, T and Saint, KM and Donnellan, S and Hammer, MP and Gillanders, BM, Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids, Conservation Genetics Resources, 6, (2) pp. 345-348. ISSN 1877-7252 (2014) [Refereed Article]

Copyright Statement

Copyright 2013 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s12686-013-0090-7

Abstract

Next generation sequencing was used to develop 15 new polymorphic microsatellite markers for the commercially and recreationally important fish mulloway (Argyrosomus japonicus). Improved knowledge of the genetic structure of the species is necessary for fisheries and environmental management. In a population of mulloway, we found that genetic variation and heterozygosity were high in most of our loci (mean number of alleles per locus = 9.46; mean heterozygosity = 0.660). To test the usefulness of our new markers for genetic research on other sciaenids, we cross amplified samples from eight other related sciaenid species, including three that form important fisheries and others that have significant conservation issues. We found that most of our new primers pairs produced scorable profiles in each of the other important sciaenids.

Item Details

Item Type:Refereed Article
Keywords:population genetics, population structure, microsatellites, life history, mulloway
Research Division:Environmental Sciences
Research Group:Environmental Science and Management
Research Field:Conservation and Biodiversity
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Wild Caught
Objective Field:Fisheries - Recreational
UTAS Author:Barnes, TC (Dr Thomas Barnes)
ID Code:126825
Year Published:2014
Web of Science® Times Cited:7
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2018-06-26
Last Modified:2018-08-20
Downloads:0

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