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Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids
Citation
Barnes, TC and Izzo, C and Bertozzi, T and Saint, KM and Donnellan, S and Hammer, MP and Gillanders, BM, Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids, Conservation Genetics Resources, 6, (2) pp. 345-348. ISSN 1877-7252 (2014) [Refereed Article]
Copyright Statement
Copyright 2013 Springer Science+Business Media Dordrecht
DOI: doi:10.1007/s12686-013-0090-7
Abstract
Next generation sequencing was used to develop 15 new polymorphic microsatellite markers for the commercially and recreationally important fish mulloway (Argyrosomus japonicus). Improved knowledge of the genetic structure of the species is necessary for fisheries and environmental management. In a population of mulloway, we found that genetic variation and heterozygosity were high in most of our loci (mean number of alleles per locus = 9.46; mean heterozygosity = 0.660). To test the usefulness of our new markers for genetic research on other sciaenids, we cross amplified samples from eight other related sciaenid species, including three that form important fisheries and others that have significant conservation issues. We found that most of our new primers pairs produced scorable profiles in each of the other important sciaenids.
Item Details
Item Type: | Refereed Article |
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Keywords: | population genetics, population structure, microsatellites, life history, mulloway |
Research Division: | Environmental Sciences |
Research Group: | Environmental management |
Research Field: | Conservation and biodiversity |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - wild caught |
Objective Field: | Fisheries - recreational freshwater |
UTAS Author: | Barnes, TC (Dr Thomas Barnes) |
ID Code: | 126825 |
Year Published: | 2014 |
Web of Science® Times Cited: | 7 |
Deposited By: | Sustainable Marine Research Collaboration |
Deposited On: | 2018-06-26 |
Last Modified: | 2018-08-20 |
Downloads: | 0 |
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