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A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis
Citation
Quirino, JP, A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis, Journal of Separation Science, 40, (24) pp. 4835-4838. ISSN 1615-9314 (2017) [Refereed Article]
Copyright Statement
Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: doi:10.1002/jssc.201700610
Abstract
A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.
Item Details
Item Type: | Refereed Article |
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Keywords: | capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Quirino, JP (Associate Professor Lito Quirino) |
ID Code: | 126597 |
Year Published: | 2017 |
Web of Science® Times Cited: | 6 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2018-06-19 |
Last Modified: | 2022-08-26 |
Downloads: | 0 |
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