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A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis


Quirino, JP, A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis, Journal of Separation Science, 40, (24) pp. 4835-4838. ISSN 1615-9314 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/jssc.201700610


A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.

Item Details

Item Type:Refereed Article
Keywords:capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:126597
Year Published:2017
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2018-06-19
Last Modified:2022-08-26

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