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A cationic β-cyclodextrin as a dynamic coating for the separationof proteins in capillary electrophoresis
journal contribution
posted on 2023-05-19, 18:54 authored by Joselito QuirinoA cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.
History
Publication title
Journal of Separation ScienceVolume
40Issue
24Pagination
4835-4838ISSN
1615-9314Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
Po Box 10 11 61, Weinheim, Germany, D-69451Rights statement
Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted