A cationic cyclodextrin was used as dynamic coating for the capillary electrophoresis of a model mixture of proteins (i.e., ubiquitin, α-lactoglobulin, cytochrome-c, and myoglobin) as positively charged species in a fused silica capillary. An interesting feature of the coating is that by simple adjustment of the concentration of cyclodextrin added into the background electrolyte, a neutral or positively charged surface, which was beneficial in preventing protein adsorption at the inner capillary wall surface, was obtained. This is t he first demonstration of a dynamic coating that yielded a neutral surface for protein separations in capillary electrophoresis. Based on electro-osmotic flow measurements, addition of 0.05 to 0.10 mg/mL quater nary β-cyclodextrin in alow pH electrolyte resulted in a neutral or positive surface (undetectable to very slow anodic electro-osmotic flow). The coating approach afforded the electrophoretic separation of the mixture of proteins at positive polarity with good repeatability and separation performance.

Item Type:	Refereed Article
Keywords:	capillary electrophoresis, cationic cyclodextrins, dynamic coating, neutral surfaces, proteins
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Quirino, JP (Associate Professor Lito Quirino)
ID Code:	126597
Year Published:	2017
Web of Science® Times Cited:	6
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2018-06-19
Last Modified:	2022-08-26
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