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Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells
Citation
Keremidarska-Markova, M and Radeva, E and Mitev, D and Hristova-Panusheva, K and Paull, B and Nesterenko, P and Sepitka, J and Junkar, I and Iglic, A and Krasteva, N, Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells, Turkish Journal of Biology, 42, (2) pp. 195-203. ISSN 1300-0152 (2018) [Refereed Article]
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Copyright Statement
© TÜBİTAK 2018
Abstract
In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.
Item Details
Item Type: | Refereed Article |
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Keywords: | detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Paull, B (Professor Brett Paull) |
UTAS Author: | Nesterenko, P (Professor Pavel Nesterenko) |
ID Code: | 126237 |
Year Published: | 2018 |
Funding Support: | Australian Research Council (DP110102046) |
Web of Science® Times Cited: | 2 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2018-05-30 |
Last Modified: | 2019-03-08 |
Downloads: | 68 View Download Statistics |
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