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Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells

Citation

Keremidarska-Markova, M and Radeva, E and Mitev, D and Hristova-Panusheva, K and Paull, B and Nesterenko, P and Sepitka, J and Junkar, I and Iglic, A and Krasteva, N, Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells, Turkish Journal of Biology, 42, (2) pp. 195-203. ISSN 1300-0152 (2018) [Refereed Article]


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© TÜBİTAK 2018

DOI: doi:10.3906/biy-1711-26

Abstract

In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.

Item Details

Item Type:Refereed Article
Keywords:detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
UTAS Author:Paull, B (Professor Brett Paull)
UTAS Author:Nesterenko, P (Professor Pavel Nesterenko)
ID Code:126237
Year Published:2018
Funding Support:Australian Research Council (DP110102046)
Web of Science® Times Cited:1
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2018-05-30
Last Modified:2019-03-08
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