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Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells
Citation
Keremidarska-Markova, M and Radeva, E and Mitev, D and Hristova-Panusheva, K and Paull, B and Nesterenko, P and Sepitka, J and Junkar, I and Iglic, A and Krasteva, N, Increased elastic modulus of plasma polymer coatings reinforced with detonation nanodiamond particles improves osteogenic differentiation of mesenchymal stem cells, Turkish Journal of Biology, 42, (2) pp. 195-203. ISSN 1300-0152 (2018) [Refereed Article]
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Copyright Statement
© TÜBİTAK 2018
Abstract
In the present study we demonstrated that composite PPHMDS/DND coatings with elastic moduli close to those of mature bone tissue (0.2–2.8 GPa) stimulated growth and osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). Composite coatings were prepared by a method of plasma polymerization (PP) where detonation nanodiamond (DND) particles in different amounts (0.1, 0.5, and 1 mg/mL) were added to hexamethyldisiloxane (HMDS) before plasma deposition. This method allows variation only in the reduced elastic modulus (Er´) with increase in the particle concentration, while the other surface properties, including surface wettability and topography, did not change. The response of hAD-MSCs to the increasing stiffness showed an effect on adhesion and osteogenic differentiation but not on cell proliferation. Matrix mineralization and cell spreading were maximized on PPHMDS/DND coatings with the highest elastic modulus (2.826 GPa), while the differences in proliferation rates among the samples were negligible. In general, PPHMDS/DND coatings provide better conditions for growth and osteogenic differentiation of hAD-MSCs in comparison to glass coverslips, confirming their suitability for osteo-integration applications. Additionally, our findings support the hypothesis that biomaterials with elasticity similar to that of the native tissue can improve the differentiation potential of mesenchymal stem cells.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | detonation nanodiamonds, organosilicone, bone implants, stiffness, cell adhesion and growth |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Separation science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the chemical sciences |
| UTAS Author: | Paull, B (Professor Brett Paull) |
| UTAS Author: | Nesterenko, P (Professor Pavel Nesterenko) |
| ID Code: | 126237 |
| Year Published: | 2018 |
| Funding Support: | Australian Research Council (DP110102046) |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2018-05-30 |
| Last Modified: | 2019-03-08 |
| Downloads: | 68 View Download Statistics |
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