University of Tasmania
Browse

File(s) under permanent embargo

Comparison of DNA extraction and quantification methods to estimate bacterial load in oral cavity

conference contribution
posted on 2023-05-24, 17:38 authored by Khadka, S, Silvana BettiolSilvana Bettiol, Anna KingAnna King, Lynette GoldbergLynette Goldberg, Sharee McCammonSharee McCammon, Crocombe, LA
Oral health is now beyond pearly white shining teeth and has been associated with overall health and quality of life. Oral cavity harbours more than 700 bacterial species, of which, around half are cultivable Estimation of microbial load and type In the oral cavity in normal and diseased state helps to understand the dynamics of host-pathogen interaction. There are studies reporting the concentration of microorganisms in the oral cavity. However, differences In terms of DNA extraction techniques and their quantitation methods affects the interpretation of results. The objective of this study is to compare commercially available DNA extraction kits and different methods of quantitation of bacterial DNA obtained from oral cavity.

Funding

Tasmanian Community Fund

History

Publication title

Molecular Microbiology Meeting 2018

Department/School

School of Health Sciences

Event title

Molecular Microbiology Meeting 2018

Event Venue

Sydney

Date of Event (Start Date)

2018-04-11

Date of Event (End Date)

2018-04-12

Repository Status

  • Restricted

Socio-economic Objectives

Dental health

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC