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Comparison of DNA extraction and quantification methods to estimate bacterial load in oral cavity


Khadka, S and Bettiol, S and King, A and Goldberg, LR and McCammon, SA and Crocombe, LA, Comparison of DNA extraction and quantification methods to estimate bacterial load in oral cavity, Molecular Microbiology Meeting 2018, 11-12 April 2018, Sydney (2018) [Conference Extract]

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Oral health is now beyond pearly white shining teeth and has been associated with overall health and quality of life. Oral cavity harbours more than 700 bacterial species, of which, around half are cultivable Estimation of microbial load and type In the oral cavity in normal and diseased state helps to understand the dynamics of host-pathogen interaction. There are studies reporting the concentration of microorganisms in the oral cavity. However, differences In terms of DNA extraction techniques and their quantitation methods affects the interpretation of results. The objective of this study is to compare commercially available DNA extraction kits and different methods of quantitation of bacterial DNA obtained from oral cavity.

Item Details

Item Type:Conference Extract
Keywords:Molecular biology, microbiology, oral microbial flora
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial genetics
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Dental health
UTAS Author:Khadka, S (Ms Sangeeta Khadka)
UTAS Author:Bettiol, S (Dr Silvana Bettiol)
UTAS Author:King, A (Professor Anna King)
UTAS Author:Goldberg, LR (Associate Professor Lyn Goldberg)
UTAS Author:McCammon, SA (Ms Sharee McCammon)
UTAS Author:Crocombe, LA (Associate Professor Leonard Crocombe)
ID Code:126082
Year Published:2018
Deposited By:Medicine
Deposited On:2018-05-22
Last Modified:2018-07-30
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