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Developmental plasticity in reptiles: insights from temperature-dependent gene expression in wall lizard embryos

Citation

Feiner, N and Rago, A and While, GM and Uller, T, Developmental plasticity in reptiles: insights from temperature-dependent gene expression in wall lizard embryos, Journal of Experimental Zoology pp. 1-11. ISSN 1932-5223 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 Wiley Periodicals, Inc.

DOI: doi:10.1002/jez.2175

Abstract

Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°CC) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20% less total RNA than those incubated at benign temperatures, presumably reflecting lower rates of transcription at cool temperature. After normalizing for differences in total amounts of input RNA, we find that approximately 50% of all transcripts show significant expression differences between the two incubation temperatures. Transcripts with the most extreme changes in expression profiles are associated with transcriptional and translational regulation and chromatin remodeling, suggesting possible epigenetic mechanisms underlying acclimation of early embryos to cool temperature. We discuss our findings in light of current advances in the use of transcriptomic data to study how individuals acclimatize and populations adapt to thermal stress.

Item Details

Item Type:Refereed Article
Keywords:embryo, epigenetics, gene expression, lizards, thermal plasticity, transcriptome
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene Expression (incl. Microarray and other genome-wide approaches)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
UTAS Author:While, GM (Dr Geoff While)
ID Code:126068
Year Published:2018
Deposited By:Zoology
Deposited On:2018-05-21
Last Modified:2019-03-05
Downloads:0

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